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J1248. MINERAL SALTS MEDIUM WITH TCE

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.000 g 0.944 16.158
2 0.500 g 0.472 2.322
3 0.200 g 0.189 1.388
4 0.300 g 0.283 5.296
5 0.300 g 0.283 3.8
6 0.015 g 0.014 0.096
7 FeCl2 solution 1.000 ml - -
8 Trace element solution 1.000 ml - -
9 Selenite-tungstate solution 1.000 ml - -
10 0.340 g 0.321 4.721
11 0.410 g 0.387 4.719
12 0.250 mg 2.4e-4 0.001
13 1000.000 ml 942.594 52321.9
14
(8%)
30.000 ml 4.893 58.244
15
(5%)
6.000 ml - -
16
(5%)
10.000 ml - -
17 60.000 μl - -
18 Trace vitamins 10.000 ml - -
Instructions:
  1. Mix components thoroughly, bring to a boil and cool down under a N2-CO2 (4:1, v/v) gas mixture. Add the following solutions: #14-16
  2. Adjust pH of the medium to 7.0-7.2, if necessary. Dispense the medium into culture vessels (e.g., 100 ml in 120 ml- serum bottles) under the same gas mixture and seal with butyl rubber stoppers or Teflon-lined stoppers. Then, anaerobically add per liter the following solution: #17
  3. Dissolve tetrachloroethene with ultrasound. Autoclave in a closed basket. Do not remove the culture vessels from autoclave before temperature of the media has reached below 70C.
  4. Finally, add per liter the following solution from an anaerobic stock (filter-sterilized): #18
Solution occurs in 89 other media (first defined in medium J187 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 70 mg 0.07 0.514
2 100 mg 0.1 -
3 6 mg 0.006 0.097
4 190 mg 0.19 -
5 2 mg 0.002 -
6 24 mg 0.024 -
7 36 mg 0.036 0.149
8 1000 ml 998.207 55408.8
Solution occurs in 87 other media (first defined in medium J187 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(7.7 M, 25%)
10.0 ml 3.17 86.943
2 1.5 g 1.5 -
3 990.0 ml 988.225 54854.8
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 69 other media (first defined in medium J431 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.4 g 0.4 10.001
2 6.0 mg 0.006 -
3 8.0 mg 0.008 -
4 1000.0 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 954.838 53001.6
NaHCO3 4.893 58.244
NaCl 0.944 16.158
MgCl2 x 6H2O 0.472 2.322
Sodium acetate 0.387 4.719
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