J1250
1250 .
METHYLOCOCCACEAE IN20 MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1250 #
ml
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 30.00 | g | 29.821 | 510.285 | |
| MgCl2 x 6 H2O | 4.20 | g | 4.175 | 20.536 | |
| MgSO4 x 7 H2O | 3.40 | g | 3.38 | 13.712 | |
| KCl | 0.33 | g | 0.328 | 4.4 | |
| CaCl2 x 2 H2O | 0.30 | g | 0.298 | 2.028 | |
| Na2HPO4 | 0.25 | g | 0.249 | 1.751 | |
| KH2PO4 | 0.10 | g | 0.099 | 0.73 | |
| NH4Cl | 0.25 | g | 0.249 | 4.646 | |
| Fe2(SO4)3 x n H2O | 0.02 | g | 0.02 | - | |
| Trace mineral solution | 3.30 | ml | - | - | |
|
CuSO4 x 5 H2O
(1 mM) |
0.10 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Mix components thoroughly, adjust pH to 5.7 with HCl and autoclave. After cooling, add the following solutions (filter-sterilized) to the medium: | |||||
| Trace vitamins | 1.00 | ml | - | - | |
|
NaHCO3
(8%) |
1.25 | ml | - | - | |
| 2 Aseptically distribute the medium into culture vessels (leave >80% volume gas phase), and seal with butyl rubber stoppers. Then, add methane (filter-sterilized) to the culture vessels to give a 30% concentration by volume in the gas phase (overpressured). Check final pH of the medium to be 6.4-6.8. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nitrilotriacetic acid | 1.500 | g | 1.5 | 7.848 | |
| MnSO4 x n H2O | 1.000 | g | 1 | - | |
| ZnSO4 x 7 H2O | 0.360 | g | 0.36 | 1.252 | |
| CoSO4 x 7 H2O | 0.180 | g | 0.18 | 1.161 | |
| Na2MoO4 x 2 H2O | 0.030 | g | 0.03 | 0.124 | |
| AlK(SO4)2 x 12 H2O | 0.020 | g | 0.02 | 0.042 | |
| H3BO3 | 0.010 | g | 0.01 | 0.162 | |
| Na2WO4 x 2 H2O | 0.025 | g | 0.025 | 0.076 | |
| NiCl2 x 5 H2O | 0.025 | g | 0.025 | 0.193 | |
| Na2SeO3 | 0.300 | mg | 3.0e-4 | - | |
| Distilled water | 1000.000 | ml | - | - | |
| 1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then, proceed to add minerals. Adjust final pH to 7.0. | |||||
Solution occurs in 220
other media (first defined in Medium J197).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Defined medium |
| Final pH: | 6.5 |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |