J1260
1260 .
LSR1 MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1260 #
ml
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.40 | g | 0.39 | 2.868 | |
| MgCl2 x 6 H2O | 0.12 | g | 0.117 | 0.576 | |
| NH4Cl | 0.10 | g | 0.098 | 1.824 | |
| K2SO4 | 4.00 | mg | 0.004 | 0.022 | |
| MnCl2 x 4 H2O | 3.00 | mg | 0.003 | - | |
| FeCl3 x 6 H2O | 5.00 | mg | 0.005 | 0.018 | |
| Na2MoO4 x 2 H2O | 5.00 | mg | 0.005 | 0.02 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Mix components thoroughly and autoclave under a microaerophilic atmosphere (1.0 - 3.0% O2 in N2). Aseptically add per liter the following solutions (filter-sterilized): | |||||
|
Sodium Thiosulfate
(1.0 M) |
10.00 | ml | - | - | |
| Trace vitamins | 10.00 | ml | - | - | |
|
NaHCO3
(8%) |
5.00 | ml | - | - | |
Solution occurs in 220
other media (first defined in Medium J197).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 29.10.21 |
| Source: | JCM |
| Medium type: | Defined medium |
| Final pH: | n.d. |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |