J1265
1265 .
GBS SALTS MEDIUM FOR THERMANAEROMONAS INYOENSIS
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1265 #
ml
Equipment needed: Filter
Autoclave
Anaerobic workbench
| 1 Mix components thoroughly, adjust pH to 7.0, distribute the medium into culture vessels (e.g., 5.0 ml in 25 ml serum bottles/Balch tubes) under a stream of N2, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter-sterilized and stored anaerobically): | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaH2PO4 | 1.380 | g | 1.342 | 11.189 | |
| NaCl | 3.000 | g | 2.918 | 49.937 | |
| NH4Cl | 0.107 | g | 0.104 | 1.946 | |
| MgSO4 x 7 H2O | 0.123 | g | 0.12 | 0.485 | |
| CaCl2 x 2 H2O | 0.015 | g | 0.015 | 0.099 | |
| KCl | 0.150 | g | 0.146 | 1.957 | |
| Na2SO4 | 0.300 | g | 0.292 | 2.055 | |
| Mineral solution | 5.000 | ml | - | - | |
| Distilled water | 1000.000 | ml | - | - | |
|
Sodium acetate
(1.0 M) |
2.000 | ml | - | - | |
|
Sodium Thiosulfate
(1.0 M) |
2.000 | ml | - | - | |
|
Sodium lactate
(1.0 M) |
5.000 | ml | - | - | |
|
NaHCO3
(*, 8.0%) |
2.000 | ml | - | - | |
|
Trace vitamins
(*) |
10.000 | ml | - | - | |
|
Na2S x 9 H2O
(5%) |
2.000 | ml | - | - | |
Solution occurs in 5
other media (first defined in Medium J976).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| EDTA | 3.7 | g | 3.7 | 12.661 | |
| FeSO4 x 7 H2O | 1.1 | g | 1.1 | 3.957 | |
| MnCl2 x 4 H2O | 98.4 | mg | 0.098 | - | |
| ZnSO4 x 7 H2O | 28.8 | mg | 0.029 | 0.1 | |
| CoCl2 x 6 H2O | 23.8 | mg | 0.024 | 0.183 | |
| CuCl2 x 2 H2O | 17.2 | mg | 0.017 | 0.101 | |
| Na2MoO4 x 2 H2O | 24.2 | mg | 0.024 | 0.1 | |
| H3BO3 | 31.0 | mg | 0.031 | 0.501 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Adjust pH to 6.0 with KOH. | |||||
| 2 Comment: Consistent growth in serum tubes requires pretreatment of butyl rubber stoppers by boiling in 1% sodium sulfide solution (1 hr) followed by boiling in distilled H2O (1 hr, twice). | |||||
Solution occurs in 220
other media (first defined in Medium J197).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Defined medium |
| Final pH: | 7.0 |
| Equipment needed: | Anaerobic workbench, Autoclave, Filter |
| Edit in Medium builder | |