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JCM 1277 . VULCANO HIGH SALT MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 18.00 g 17.699 302.86
MgCl2 x 2 H2O 1.00 g 0.983 7.492
MgSO4 x 7 H2O 1.00 g 0.983 3.989
KCl 0.30 g 0.295 3.957
Na2SO4 1.00 g 0.983 6.923
(NH4)2SO4 0.30 g 0.295 2.232
K2HPO4 0.05 g 0.049 0.282
KH2PO4 0.05 g 0.049 0.361
CaCl2 x 2 H2O 0.02 g 0.02 0.134
FeCl2 x 4 H2O 0.10 g 0.098 0.495
Iron(III) citrate 0.10 g 0.098 0.401
L-Cysteine HCl x H2O 0.50 g 0.492 -
MES 1.95 g 1.917 9.821
Resazurin 1.00 mg 9.8e-4 0.004
Trace element solution 1.00 ml - -
Se-Ni-W solution 1.00 ml - -
Distilled water 1000.00 ml - -
1 Mix components thoroughly, adjust pH to 6.3 and autoclave under a N2-CO2 (4:1, v/v) gas atmosphere. After cooling, add the following solutions from anaerobic stocks (autoclaved):
Yeast extract
(10%)
5.00 ml - -
Sodium fumarate
(1.0 M)
10.00 ml - -
2 Comment:
3 For JCM 39183, add 1.0 M glucose solution (final 10 mM), instead of sodium fumarate solution, and adjust pH to 5.5.
Solution occurs in 38 other media (first defined in Medium J439).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
12.5 ml - -
FeSO4 x 7 H2O 2.1 g 2.1 7.554
H3BO3 30.0 mg 0.03 0.485
MnCl2 x 4 H2O 100.0 mg 0.1 -
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
ZnSO4 x 7 H2O 144.0 mg 0.144 0.501
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 987.0 ml - -
Solution occurs in 1 other media (first defined in Medium J1243).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2SeO3 10 mg 0.1 -
NiCl2 x 6 H2O 20 mg 0.2 1.543
Na2WO4 x 2 H2O 30 mg 0.3 0.91
Distilled water 100 ml - -

Metadata

Media status: Inofficial medium
Source: JCM
Medium type: Complex medium
Final pH: 5.5
Equipment needed: Anaerobic workbench, Autoclave

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 17.699 302.86
MES 1.917 9.821
MgCl2 x 2 H2O 0.983 7.492
Na2SO4 0.983 6.923
MgSO4 x 7 H2O 0.983 3.989
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