J1277
1277 .
VULCANO HIGH SALT MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1277 #
ml
Equipment needed: Autoclave
Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 18.00 | g | 17.699 | 302.86 | |
| MgCl2 x 2 H2O | 1.00 | g | 0.983 | 7.492 | |
| MgSO4 x 7 H2O | 1.00 | g | 0.983 | 3.989 | |
| KCl | 0.30 | g | 0.295 | 3.957 | |
| Na2SO4 | 1.00 | g | 0.983 | 6.923 | |
| (NH4)2SO4 | 0.30 | g | 0.295 | 2.232 | |
| K2HPO4 | 0.05 | g | 0.049 | 0.282 | |
| KH2PO4 | 0.05 | g | 0.049 | 0.361 | |
| CaCl2 x 2 H2O | 0.02 | g | 0.02 | 0.134 | |
| FeCl2 x 4 H2O | 0.10 | g | 0.098 | 0.495 | |
| Iron(III) citrate | 0.10 | g | 0.098 | 0.401 | |
| L-Cysteine HCl x H2O | 0.50 | g | 0.492 | - | |
| MES | 1.95 | g | 1.917 | 9.821 | |
| Resazurin | 1.00 | mg | 9.8e-4 | 0.004 | |
| Trace element solution | 1.00 | ml | - | - | |
| Se-Ni-W solution | 1.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Mix components thoroughly, adjust pH to 6.3 and autoclave under a N2-CO2 (4:1, v/v) gas atmosphere. After cooling, add the following solutions from anaerobic stocks (autoclaved): | |||||
|
Yeast extract
(10%) |
5.00 | ml | - | - | |
|
Sodium fumarate
(1.0 M) |
10.00 | ml | - | - | |
| 2 Comment: | |||||
| 3 For JCM 39183, add 1.0 M glucose solution (final 10 mM), instead of sodium fumarate solution, and adjust pH to 5.5. | |||||
Solution occurs in 38
other media (first defined in Medium J439).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
|
HCl
(25%) |
12.5 | ml | - | - |
| FeSO4 x 7 H2O | 2.1 | g | 2.1 | 7.554 |
| H3BO3 | 30.0 | mg | 0.03 | 0.485 |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | - |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 |
| ZnSO4 x 7 H2O | 144.0 | mg | 0.144 | 0.501 |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 |
| Distilled water | 987.0 | ml | - | - |
Solution occurs in 1
other media (first defined in Medium J1243).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Na2SeO3 | 10 | mg | 0.1 | - |
| NiCl2 x 6 H2O | 20 | mg | 0.2 | 1.543 |
| Na2WO4 x 2 H2O | 30 | mg | 0.3 | 0.91 |
| Distilled water | 100 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | 5.5 |
| Equipment needed: | Anaerobic workbench, Autoclave |
| Edit in Medium builder | |