1277 .
VULCANO HIGH SALT MEDIUM
What is this page?
Equipment needed:
Anaerobic workbench
Autoclave
Final pH:
5.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl | 18.00 | g | ||
|
|
MgCl2 x 2 H2O | 1.00 | g | ||
|
|
MgSO4 x 7 H2O | 1.00 | g | ||
|
|
KCl | 0.30 | g | ||
|
|
Na2SO4 | 1.00 | g | ||
|
|
(NH4)2SO4 | 0.30 | g | ||
|
|
K2HPO4 | 0.05 | g | ||
|
|
KH2PO4 | 0.05 | g | ||
|
|
CaCl2 x 2 H2O | 0.02 | g | ||
|
|
FeCl2 x 4 H2O | 0.10 | g | ||
|
|
Iron(III) citrate | 0.10 | g | ||
|
|
L-Cysteine HCl x H2O | 0.50 | g | ||
|
|
MES | 1.95 | g | ||
|
|
Resazurin | 1.00 | mg | ||
|
|
Trace element solution | 1.00 | ml | ||
|
|
Se-Ni-W solution | 1.00 | ml | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Mix components thoroughly, adjust pH to 6.3 and autoclave under a N2-CO2 (4:1, v/v) gas atmosphere. After cooling, add the following solutions from anaerobic stocks (autoclaved): | |||||
|
|
Yeast extract
(10%) |
5.00 | ml | ||
|
|
Sodium fumarate
(1.0 M) |
10.00 | ml | ||
| 2 Comment: | |||||
| 3 For JCM 39183, add 1.0 M glucose solution (final 10 mM), instead of sodium fumarate solution, and adjust pH to 5.5. | |||||
Trace element solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
HCl
(25%) |
12.5 | ml |
|
|
FeSO4 x 7 H2O | 2.1 | g |
|
|
H3BO3 | 30.0 | mg |
|
|
MnCl2 x 4 H2O | 100.0 | mg |
|
|
CoCl2 x 6 H2O | 190.0 | mg |
|
|
NiCl2 x 6 H2O | 24.0 | mg |
|
|
CuCl2 x 2 H2O | 2.0 | mg |
|
|
ZnSO4 x 7 H2O | 144.0 | mg |
|
|
Na2MoO4 x 2 H2O | 36.0 | mg |
|
|
Distilled water | 987.0 | ml |
Se-Ni-W solution #
100
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Na2SeO3 | 10 | mg |
|
|
NiCl2 x 6 H2O | 20 | mg |
|
|
Na2WO4 x 2 H2O | 30 | mg |
|
|
Distilled water | 100 | ml |