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JCM 1278 . MIZ13 MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
Modified Wolfe's mineral solution 1000.00 ml - -
Trace minerals 10.00 ml - -
MES 1.95 g 1.893 9.697
NaHCO3 0.83 g 0.806 9.592
Sodium acetate 1.36 g 1.32 16.096
Sodium pyruvate 1.10 g 1.068 9.705
Sodium fumarate 3.20 g 3.107 19.413
1 Mix components thoroughly and adjust pH 6.2. Autoclave under a N2 atmosphere. After cooling, add the following solutions anaerobically and aseptically (autoclaved or *filter-sterilized and stored under a N2 atmosphere):
Trace vitamins
(*)
10.00 ml - -
L-Cysteine HCl x H2O
(5%)
10.00 ml - -
Solution occurs in 3 other media (first defined in Medium J628).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 1.00 g 1 18.695
MgSO4 x 7 H2O 0.20 g 0.2 0.811
CaCl2 x 2 H2O 0.10 g 0.1 0.68
KH2PO4 0.05 g 0.05 0.367
Distilled water 1000.00 ml - -
Solution occurs in 97 other media (first defined in Medium J151).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x n H2O 0.50 g 0.5 -
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.10 g 0.1 0.645
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 0.01 g 0.01 0.039
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 -
Thiamine HCl 5.0 mg 0.005 -
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Media status: Inofficial medium
Source: JCM
Medium type: Defined medium
Final pH: n.d.
Equipment needed: Anaerobic workbench, Autoclave, Filter

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Sodium fumarate 3.107 19.413
MES 1.893 9.697
Sodium acetate 1.32 16.096
Sodium pyruvate 1.068 9.705
NH4Cl 0.971 18.151
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