1278 .
MIZ13 MEDIUM
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Equipment needed:
Anaerobic workbench
Autoclave
Filter
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Modified Wolfe's mineral solution | 1000.00 | ml | ||
|
|
Trace minerals | 10.00 | ml | ||
|
|
MES | 1.95 | g | ||
|
|
NaHCO3 | 0.83 | g | ||
|
|
Sodium acetate | 1.36 | g | ||
|
|
Sodium pyruvate | 1.10 | g | ||
|
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Sodium fumarate | 3.20 | g | ||
| 1 Mix components thoroughly and adjust pH 6.2. Autoclave under a N2 atmosphere. After cooling, add the following solutions anaerobically and aseptically (autoclaved or *filter-sterilized and stored under a N2 atmosphere): | |||||
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|
Trace vitamins
(*) |
10.00 | ml | ||
|
|
L-Cysteine HCl x H2O
(5%) |
10.00 | ml | ||
Modified Wolfe's mineral solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
NH4Cl | 1.00 | g |
|
|
MgSO4 x 7 H2O | 0.20 | g |
|
|
CaCl2 x 2 H2O | 0.10 | g |
|
|
KH2PO4 | 0.05 | g |
|
|
Distilled water | 1000.00 | ml |
Trace minerals #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Nitrilotriacetic acid | 1.50 | g | ||
|
|
MgSO4 x 7 H2O | 3.00 | g | ||
|
|
MnSO4 x n H2O | 0.50 | g | ||
|
|
NaCl | 1.00 | g | ||
|
|
FeSO4 x 7 H2O | 0.10 | g | ||
|
|
CoSO4 x 7 H2O | 0.10 | g | ||
|
|
CaCl2 x 2 H2O | 0.10 | g | ||
|
|
ZnSO4 x 7 H2O | 0.10 | g | ||
|
|
CuSO4 x 5 H2O | 0.01 | g | ||
|
|
AlK(SO4)2 | 0.01 | g | ||
|
|
H3BO3 | 0.01 | g | ||
|
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Na2MoO4 x 2 H2O | 0.01 | g | ||
|
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Distilled water | 1000.00 | ml | ||
| 1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0. | |||||
Trace vitamins #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 2.0 | mg |
|
|
Folic acid | 2.0 | mg |
|
|
Pyridoxine hydrochloride | 10.0 | mg |
|
|
Thiamine HCl | 5.0 | mg |
|
|
Riboflavin | 5.0 | mg |
|
|
Nicotinic acid | 5.0 | mg |
|
|
Calcium pantothenate | 5.0 | mg |
|
|
Vitamin B12 | 0.1 | mg |
|
|
p-Aminobenzoic acid | 5.0 | mg |
|
|
Lipoic acid | 5.0 | mg |
|
|
Distilled water | 1000.0 | ml |