J1283
1283 .
SIUC-3 MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1283 #
ml
Equipment needed: Anaerobic workbench
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| HEPES | 3.60 | g | 3.561 | 14.942 | |
| Na2SO4 | 3.24 | g | 3.205 | 22.562 | |
| K2HPO4 | 0.17 | g | 0.168 | 0.965 | |
| MgCl2 x 6 H2O | 0.40 | g | 0.396 | 1.946 | |
| KCl | 0.50 | g | 0.495 | 6.634 | |
| NH4Cl | 0.30 | g | 0.297 | 5.548 | |
| NaHCO3 | 0.25 | g | 0.247 | 2.944 | |
| H3BO3 | 6.00 | mg | 0.006 | 0.096 | |
| Wolfe's mineral solution | 1.00 | ml | - | - | |
| Sodium formate | 0.68 | g | 0.673 | 9.89 | |
| Yeast extract | 0.10 | g | 0.099 | - | |
| Resazurin | 0.20 | mg | 2.0e-4 | 8.6e-4 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Mix components thoroughly and adjust pH to 6.5. Distribute the medium into culture vessels (e.g., 25 ml in 120 ml-serum bottles) under a stream of a N2 gas, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solution (autoclaved) : | |||||
|
Na2S x 9 H2O
(5%) |
10.00 | ml | - | - | |
Solution occurs in 7
other media (first defined in Medium J265).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Trace minerals | 1000.00 | ml | - | - |
| NiCl2 x 6 H2O | 0.02 | g | 0.02 | 0.154 |
| Na2SeO3 | 1.00 | mg | 1.0e-3 | - |
| Na2WO4 x 2 H2O | 0.01 | g | 0.01 | 0.03 |
Solution occurs in 97
other media (first defined in Medium J151).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
| MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
| MnSO4 x n H2O | 0.50 | g | 0.5 | - | |
| NaCl | 1.00 | g | 1 | 17.112 | |
| FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
| CoSO4 x 7 H2O | 0.10 | g | 0.1 | 0.645 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
| CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
| AlK(SO4)2 | 0.01 | g | 0.01 | 0.039 | |
| H3BO3 | 0.01 | g | 0.01 | 0.162 | |
| Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | 6.5 |
| Equipment needed: | Anaerobic workbench, Autoclave |
| Edit in Medium builder | |