MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ

JCM 1288 . NATRONOANAEROARCHAEA MEDIUM WITH YEAST EXTRACT AND FORMATE

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1288 #

ml
Equipment needed: Anaerobic workbench Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Base salt medium 750.0 ml - -
Base soda medium 250.0 ml - -
1 Mix two media autoclaved separately. Aseptically add the following solutions (autoclaved or *filter-sterilized):
MgSO4
(1 M) 		1.0 ml - -
Trace element solution 1.0 ml - -
Selenite-tungstate solution
(*) 		1.0 ml - -
Trace vitamins
(*) 		5.0 ml - -
2 To complete the medium, aseptically and anaerobically distribute the medium into culture vessels containing steam-sterilized sulfur (see below, approximately 5.0 g/L) under an argon gas stream, and seal with butyl rubber stoppers. Then, add per liter the following solutions (autoclaved or *filter-sterilized):
Yeast extract
(10%) 		5.0 ml - -
Sodium formate
(*, 5.0 M) 		10.0 ml - -
Na2S x 9 H2O
(5%) 		2.5 ml - -

Sterilization of sulfur #

Solution occurs in 4 other media (first defined in Medium J1079).
Equipment needed: Autoclave
1 Steam sulfur for 3 hr, or autoclave at 105C for 1 hr, on each of 3 successive days.

Base salt medium #

Solution occurs in 6 other media (first defined in Medium J1207).
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 240.0 g - -
KCl 5.0 g - -
K2HPO4 2.5 g - -
NH4Cl 0.5 g - -
1 Add components to distilled water and bring volume to 1.0 L. Adjust pH to 7.0 with 0.5 M K2HPO4 and autoclave.

Base soda medium #

Solution occurs in 4 other media (first defined in Medium J1207).
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2CO3 190 g - -
NaHCO3 30 g - -
NaCl 16 g - -
K2HPO4 1 g - -
1 Add components to distilled water and bring volume to 1.0 L. Autoclave.

Trace element solution #

1000
Solution occurs in 13 other media (first defined in Medium J1079).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5.00 g 5 17.109
FeSO4 x 7 H2O 2.00 g 2 7.194
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 -
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 Adjust pH to 3.6.

Selenite-tungstate solution #

1000
Solution occurs in 71 other media (first defined in Medium J431).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.4 g 0.4 10.001
Na2SeO3 x 5 H2O 6.0 mg 0.006 0.023
Na2WO4 x 2 H2O 8.0 mg 0.008 0.024
Distilled water 1000.0 ml - -

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 29.10.21
Source: JCM
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Anaerobic workbench, Autoclave, Filter
Edit in Medium builder

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
EDTA 0.005 0.017
FeSO4 x 7 H2O 0.002 0.007
NaOH 3.9e-4 0.01
H3BO3 2.9e-4 0.005
CoCl2 x 6 H2O 1.9e-4 0.002
Show more
CSV
JSON