J1289
1289 .
NATRONIELLA MEDIUM FOR ANB-PHB2
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1289 #
ml
Equipment needed: Filter
Autoclave
Anaerobic workbench
| 1 To complete the medium, aseptically and anaerobically distribute the medium into culture vessels under an argon gas stream, and seal with butyl rubber stoppers. Then, add per liter the following solutions (autoclaved or *filter-sterilized): | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2CO3 | 64.0 | g | 2666.67 | 25160.1 | |
| NaHCO3 | 40.0 | g | 1666.67 | 19839.6 | |
| NaCl | 18.0 | g | 750 | 12833.7 | |
| K2HPO4 | 1.0 | g | 41.667 | 239.222 | |
| 2 Add components to distilled water and bring volume to 1.0 L. Adjust pH to 9.6 with 6N HCl. Autoclave. After cooling, aseptically add the following solutions (autoclaved or *filter-sterilized): | |||||
|
MgCl2
(1 M) |
1.0 | ml | - | - | |
|
NH4Cl
(1 N) |
4.0 | ml | - | - | |
| Trace element solution | 1.0 | ml | - | - | |
|
Selenite-tungstate solution
(*) |
1.0 | ml | - | - | |
|
Trace vitamins
(*) |
1.0 | ml | - | - | |
|
Yeast extract
(1.0%) |
2.0 | ml | - | - | |
|
Sodium crotonate
(*, 2.0 M) |
10.0 | ml | - | - | |
|
Na2S x 9 H2O
(5%) |
2.5 | ml | - | - | |
|
Sodium dithionite
(*, 0.1%, in 1 M NaHCO3) |
2.0 | ml | - | - | |
Solution occurs in 13
other media (first defined in Medium J1079).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| EDTA | 5.00 | g | 5 | 17.109 | |
| FeSO4 x 7 H2O | 2.00 | g | 2 | 7.194 | |
| ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
| MnCl2 x 4 H2O | 0.03 | g | 0.03 | - | |
| H3BO3 | 0.30 | g | 0.3 | 4.851 | |
| CoCl2 x 6 H2O | 0.20 | g | 0.2 | 1.54 | |
| CuCl2 x 2 H2O | 0.01 | g | 0.01 | 0.059 | |
| NiCl2 x 6 H2O | 0.02 | g | 0.02 | 0.154 | |
| Na2MoO4 x 2 H2O | 0.03 | g | 0.03 | 0.124 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 3.6. | |||||
Solution occurs in 71
other media (first defined in Medium J431).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| NaOH | 0.4 | g | 0.4 | 10.001 |
| Na2SeO3 x 5 H2O | 6.0 | mg | 0.006 | 0.023 |
| Na2WO4 x 2 H2O | 8.0 | mg | 0.008 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Solution occurs in 220
other media (first defined in Medium J197).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | 9.6 |
| Equipment needed: | Anaerobic workbench, Autoclave, Filter |
| Edit in Medium builder | |