Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
1 Mix components thoroughly and adjust pH to 7.2 with NaOH. Bring to a boil, then cool down and distribute into culture vessels under a N2 gas stream, seal with butyl rubber stoppers, and autoclave. After cooling, aseptically and anaerobically add the following solution (autoclaved): | |||||
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KH2PO4 | 1.50 | g | 1.473 | 10.828 | |
K2HPO4 | 2.90 | g | 2.849 | 16.355 | |
Urea | 2.10 | g | 2.063 | 34.349 | |
MOPS | 10.00 | g | 9.823 | 46.94 | |
Yeast extract | 6.00 | g | 5.894 | - | |
Trisodium citrate x 2 H2O | 2.90 | g | 2.849 | - | |
L-Cysteine HCl x H2O | 0.25 | g | 0.246 | - | |
Resazurin | 1.00 | mg | 9.8e-4 | 0.004 | |
Distilled water | 1000.00 | ml | - | - | |
GS2 salt solution | 10.00 | ml | - | - | |
Cellobiose
(0.2 M) |
8.00 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
MgCl2 x 6 H2O | 100.0 | g | 100 | 491.877 |
CaCl2 x 2 H2O | 15.0 | g | 15 | 102.03 |
FeSO4
(1.25%) |
0.1 | ml | - | - |
Distilled water | 1000.0 | ml | - | - |
Last modified: | 22.02.22 |
Source: | JCM |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 7.2 |
Equipment needed: | Anaerobic workbench, Autoclave |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
MOPS | 9.823 | 46.94 |
Yeast extract | 5.894 | - |
K2HPO4 | 2.849 | 16.355 |
Trisodium citrate x 2 H2O | 2.849 | - |
Urea | 2.063 | 34.349 |
Show more |