J1292
1292 .
MQ116 MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1292 #
ml
Equipment needed: Anaerobic workbench
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 0.3 | g | 0.294 | 5.028 | |
| KCl | 0.1 | g | 0.098 | 1.314 | |
| NH4Cl | 0.3 | g | 0.294 | 5.493 | |
| KH2PO4 | 0.3 | g | 0.294 | 2.159 | |
| Na2HPO4 | 0.3 | g | 0.294 | 2.07 | |
| MgCl2 x 6 H2O | 0.2 | g | 0.196 | 0.964 | |
| CaCl2 x 2 H2O | 0.1 | g | 0.098 | 0.666 | |
| Yeast extract | 5.0 | g | 4.897 | - | |
| Beef extract | 3.0 | g | 2.938 | - | |
| Trace element solution | 1.0 | ml | - | - | |
| Resazurin | 0.5 | mg | 4.9e-4 | 0.002 | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.49 | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Mix components thoroughly and adjust pH to 7.0. Distribute the medium into culture vessels (e.g., 20 ml medium in 60 ml serum bottle) under a N2 gas atmosphere, seal with butyl rubber stoppers and autoclave.After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved and stored under N2) | |||||
|
Sodium pyruvate
(1.0 M) |
10.0 | ml | - | - | |
|
Rumen fluid
, clarified
(see Medium No. 266) |
10.0 | ml | - | - | |
Solution occurs in 7
other media (first defined in Medium J284).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nitrilotriacetic acid | 12.800 | g | 12.8 | 66.967 | |
| FeCl3 x 6 H2O | 1.350 | g | 1.35 | 4.995 | |
| MnCl2 x 4 H2O | 0.100 | g | 0.1 | - | |
| CoCl2 x 6 H2O | 0.024 | g | 0.024 | 0.185 | |
| CaCl2 x 2 H2O | 0.100 | g | 0.1 | 0.68 | |
| ZnCl2 | 0.100 | g | 0.1 | 0.734 | |
| CuCl2 x 2 H2O | 0.025 | g | 0.025 | 0.147 | |
| H3BO3 | 0.010 | g | 0.01 | 0.162 | |
| Na2MoO4 x 2 H2O | 0.024 | g | 0.024 | 0.099 | |
| NiCl2 x 6 H2O | 0.120 | g | 0.12 | 0.926 | |
| Distilled water | 1000.000 | ml | - | - | |
| 1 Dissolve nitrilotriacetic acid first in 200 ml of distilled water and adjust pH to 6.5 with KOH. Then add the remaining salts and bring volume to 1.0 L. Readjust pH to 7.0 with KOH. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Equipment needed: | Anaerobic workbench, Autoclave |
| Edit in Medium builder | |