J1295
1295 .
ALKALINE CLOSTRIDIAL NUTRIENT MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1295 #
ml
Equipment needed: Autoclave
| 1 Solution B: | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Yeast extract | 3.0 | g | 3 | - | |
| Peptone | 5.0 | g | 5 | - | |
| Beef extract | 10.0 | g | 10 | - | |
| Glucose | 5.0 | g | 5 | 27.753 | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.5 | - | |
| NaCl | 5.0 | g | 5 | 85.558 | |
| Sodium acetate | 3.0 | g | 3 | 36.572 | |
|
Starch
(soluble) |
1.0 | g | 1 | - | |
| Distilled water | 900.0 | ml | - | - | |
| Na2CO3 | 7.0 | g | 7 | 66.045 | |
| NaHCO3 | 2.9 | g | 2.9 | 34.521 | |
| Distilled water | 100.0 | ml | - | - | |
| 2 Solution A: | |||||
| 3 Adjust pH to 6.5-7.0. | |||||
| 4 Separately autoclave Solutions A and B, and combine after cooling. Cultivate under a static culture condition or a N2 atmosphere. For preparation of solid medium, add 10.0 g gellan gum in Solution A (900 ml). After autoclaving, mix the two solutions while being hot (60 to 70 C) and pour into test tubes or petri dishes. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | 6.8 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |