J1297
1297 .
MMJ/THI MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1297 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 30.00 | g | 29.674 | 507.762 | |
| MgCl2 x 6 H2O | 4.20 | g | 4.154 | 20.434 | |
| MgSO4 x 7 H2O | 3.40 | g | 3.363 | 13.644 | |
| KCl | 0.33 | g | 0.326 | 4.378 | |
| NH4Cl | 0.25 | g | 0.247 | 4.623 | |
| K2HPO4 | 0.14 | g | 0.138 | 0.795 | |
| CaCl2 x 2 H2O | 0.14 | g | 0.138 | 0.942 | |
| NiCl2 x 6 H2O | 0.50 | mg | 4.9e-4 | 0.004 | |
| Na2SeO3 | 0.30 | mg | 3.0e-4 | - | |
| Fe(NH4)2(SO4)2 x 6 H2O | 0.01 | g | 0.01 | - | |
| Trace minerals | 10.00 | ml | - | - | |
| PIPES | 6.00 | g | 5.935 | 19.625 | |
|
Vitamin solution
(see Medium No. 197) |
1.00 | ml | - | - | |
| Na2S2O3 x 5 H2O | 5.00 | g | 4.946 | 19.927 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 7.0. | |||||
Solution occurs in 97
other media (first defined in Medium J151).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
| MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
| MnSO4 x n H2O | 0.50 | g | 0.5 | - | |
| NaCl | 1.00 | g | 1 | 17.112 | |
| FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
| CoSO4 x 7 H2O | 0.10 | g | 0.1 | 0.645 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
| CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
| AlK(SO4)2 | 0.01 | g | 0.01 | 0.039 | |
| H3BO3 | 0.01 | g | 0.01 | 0.162 | |
| Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Defined medium |
| Final pH: | 7.0 |
| Edit in Medium builder | |