1298 .
ABSS2
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Equipment needed:
Anaerobic workbench
Autoclave
Filter
Final pH:
7.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl | 11.8 | g | ||
|
|
MgCl2 x 6 H2O | 2.2 | g | ||
|
|
CaCl2 x 2 H2O | 0.3 | g | ||
|
|
KCl | 0.4 | g | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Mix components and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter-sterilized): | |||||
|
|
NaHCO3
(*, 1 M) |
3.0 | ml | ||
|
|
NH4Cl
(100 mM) |
20.0 | �l | ||
|
|
Na2HPO4
(10 mM) |
100.0 | �l | ||
|
|
SL-7 trace element solution | 100.0 | �l | ||
|
|
Vitamin solution
(*) |
420.0 | �l | ||
|
|
Na2S2O3
(300 mM) |
10.0 | ml | ||
|
|
MnO2
(500 mM) |
10.0 | ml | ||
| 2 Adjust pH to 7.5 by bubbling with a N2-CO2 (4:1, v/v) gas mixture. | |||||
Vitamin solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Vitamin B12 | 10 | mg |
|
|
Inositol | 10 | mg |
|
|
Biotin | 10 | mg |
|
|
Folic acid | 10 | mg |
|
|
p-Aminobenzoic acid | 100 | mg |
|
|
Nicotinic acid | 1 | g |
|
|
DL-Calcium pantothenate | 1 | g |
|
|
Thiamine HCl | 2 | g |
|
|
Distilled water | 1000 | ml |
SL-7 trace element solution #
1010
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
HCl
(7.7 M, 25%) |
10.0 | ml | ||
|
|
FeCl2 x 4 H2O | 1.5 | g | ||
|
|
CoCl2 x 6 H2O | 190.0 | mg | ||
|
|
MnCl2 x 4 H2O | 100.0 | mg | ||
|
|
ZnCl2 | 70.0 | mg | ||
|
|
H3BO3 | 62.0 | mg | ||
|
|
Na2MoO4 x 2 H2O | 36.0 | mg | ||
|
|
NiCl2 x 6 H2O | 24.0 | mg | ||
|
|
CuCl2 x 2 H2O | 17.0 | mg | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Initially, dissolve FeCl2 x 4H2O in 25% HCl, then add distilled water followed by the other components. | |||||