Loading ...

J13. BL AGAR (GLUCOSE BLOOD LIVER AGAR)

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(Oxoid)
3.0 g 2.857 -
2
(BD-Difco)
10.0 g 9.524 -
3
(BD-BBL)
5.0 g 4.762 -
4
(BD-BBL)
3.0 g 2.857 -
5
(BD-Difco)
5.0 g 4.762 -
6
(see below)
150.0 ml - -
7 10.0 g 9.524 52.864
8 0.5 g 0.476 -
9 Solution A 10.0 ml - -
10 Solution B 5.0 ml - -
11 1.0 g 0.952 0.728
12
(BD-Difco)
15.0 g 14.286 -
13
(5%)
10.0 ml - -
14 50.0 ml - -
15 825.0 ml 784.305 43535.5
Instructions:
  1. Adjust pH to 7.2.
  2. To prepare liver extract, put 10 g liver powder in 170 ml water, keep at 50 to 60C for 1 hr, boil for 5 min, adjust pH to 7.2 and filter.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 10 g 100 574.132
2 10 g 100 734.835
3 100 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 4.0 g 40 162.285
2 0.2 g 2 34.222
3 0.2 g 2 7.194
4 0.2 g 2 -
5 100.0 ml 998.207 55408.8
Instructions:
  1. Mix ingredients except horse blood and adjust pH to 7.2. After autoclaving, cool to 50C, and aseptically add 50.0 ml of horse blood.
  2. Comment: Commercial BL agar (Nissui) is also available.

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 758

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 798.565 44327.1
Bacto Agar 14.286 -
Glucose 9.524 52.864
Proteose peptone No. 3 9.524 -
Yeast extract 4.762 -
Show more

Similar media

J460. FLUID THIOGLYCOLLATE MEDIUM 60 %
J363. PY4S AGAR 58 %
J674. PYG MEDIUM (F) 58 %
J209. M98-5 58 %
J458. B MEDIUM 56 %

Latest comments

No comments for this medium.