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J1301. SPIROCHAETEA NS MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.00 g 0.965 11.357
2 0.20 g 0.193 1.108
3 0.25 g 0.241 4.511
4 0.40 g 0.386 1.566
5 0.50 g 0.483 1.736
6 0.11 g 0.106 0.722
7 Trace mineral solution 1.00 ml - -
8 1.00 mg 9.7e-4 0.004
9 1000.00 ml 963.52 53483.4
10 Trace vitamins
(*)
20.00 ml - -
11
(1.0 M)
10.00 ml - -
12
(5%)
5.00 ml - -
Instructions:
  1. Mix components thoroughly, adjust pH to 8.0, bring to a boil for a few seconds and cool down under a stream of N2. Distribute the medium into culture vessels (e.g., 10 ml in Hungate tubes) under a N2 gas stream, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions from sterile anaerobic stocks (autoclaved or *filter-sterilized): #10-12
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 4 other media (first defined in medium J1084 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Trace minerals 1000.00 ml - -
2 0.03 g 0.03 -
3 0.30 mg 3.0e-4 -
4 0.40 mg 4.0e-4 -

Metadata

Media status: Inofficial medium
Source: JCM
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 983.754 54606.6
NaNO3 0.965 11.357
FeSO4 x 7H2O 0.483 1.736
MgSO4 x 7H2O 0.389 1.578
NH4Cl 0.241 4.511
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