J1301
1301 .
SPIROCHAETEA NS MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1301 #
ml
Equipment needed: Autoclave
Hungate tubes
Anaerobic workbench
Filter
| 1 Mix components thoroughly, adjust pH to 8.0, bring to a boil for a few seconds and cool down under a stream of N2. Distribute the medium into culture vessels (e.g., 10 ml in Hungate tubes) under a N2 gas stream, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions from sterile anaerobic stocks (autoclaved or *filter-sterilized): | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaNO3 | 1.00 | g | 0.965 | 11.357 | |
| K2HPO4 | 0.20 | g | 0.193 | 1.108 | |
| NH4Cl | 0.25 | g | 0.241 | 4.511 | |
| MgSO4 x 7 H2O | 0.40 | g | 0.386 | 1.566 | |
| FeSO4 x 7 H2O | 0.50 | g | 0.483 | 1.736 | |
| CaCl2 x 2 H2O | 0.11 | g | 0.106 | 0.722 | |
| Trace mineral solution | 1.00 | ml | - | - | |
| Resazurin | 1.00 | mg | 9.7e-4 | 0.004 | |
| Distilled water | 1000.00 | ml | - | - | |
|
Trace vitamins
(*) |
20.00 | ml | - | - | |
|
Maltose
(1.0 M) |
10.00 | ml | - | - | |
|
Na2S x 9 H2O
(5%) |
5.00 | ml | - | - | |
Solution occurs in 4
other media (first defined in Medium J1084).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Trace minerals | 1000.00 | ml | - | - |
| NiCl2 x 6 H2O | 0.03 | g | 0.03 | 0.231 |
| Na2SeO3 x 5 H2O | 0.30 | mg | 3.0e-4 | 0.001 |
| Na2WO4 x 2 H2O | 0.40 | mg | 4.0e-4 | - |
Solution occurs in 220
other media (first defined in Medium J197).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Defined medium |
| Final pH: | 8.0 |
| Equipment needed: | Anaerobic workbench, Autoclave, Filter, Hungate tubes |
| Edit in Medium builder | |