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J1302. SEA SALTS YTG MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.492 -
2 1.0 g 0.983 -
3 2.5 g 2.458 13.645
4
(Sigma-Aldrich)
30.0 g 29.499 -
5 Trace minerals 1.0 ml - -
6
(0.1%)
1.0 ml - -
7 0.2 g 0.197 1.12
8 0.5 mg 4.9e-4 0.002
9 1000.0 ml 981.521 54482.6
10 Trace vitamins 10.0 ml - -
11
(1M)
5.0 ml 11.529 72.918
Instructions:
  1. Mix components and adjust pH to 7.0. Dispense the medium into culture vessels under a N2 gas atmosphere (e.g., 10 ml medium in Hungate tubes), seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobicallly add per liter the following solutions (filter-sterilized): #10-11
Solution occurs in 88 other media (first defined in medium J151 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.50 g 1.5 7.848
2 3.00 g 3 12.171
3 0.50 g 0.5 -
4 1.00 g 1 17.111
5 0.10 g 0.1 0.36
6 0.10 g 0.1 -
7 0.10 g 0.1 0.68
8 0.10 g 0.1 -
9 0.01 g 0.01 -
10 0.01 g 0.01 0.039
11 0.01 g 0.01 0.162
12 0.01 g 0.01 0.041
13 1000.00 ml 998.207 55408.8
Instructions:
  1. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 992.318 55081.9
Sea salts 29.499 -
Sodium Thiosulfate 11.529 72.918
Glucose 2.458 13.645
Tryptone 0.983 -
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