MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ

JCM 1304 . FRESHWATER MEDIUM WITH RHAMNOSE

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1304 #

ml
Equipment needed: Anaerobic workbench Filter Hungate tubes Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 1.00 g 0.992 16.976
MgCl2 x 6 H2O 0.40 g 0.397 1.952
CaCl2 x 2 H2O 0.15 g 0.149 1.012
NH4Cl 0.25 g 0.248 4.637
KH2PO4 0.20 g 0.198 1.458
KCl 0.50 g 0.496 6.654
Resazurin 0.50 mg 5.0e-4 0.002
Distilled water 955.00 ml - -
1 Mix components thoroughly, adjust pH to 7.2-7.4 and autoclave under a N2-CO2 (80:20, v/v) gas mixture. After cooling, while gassing with the same gas mixture, add the following solutions from sterile anaerobic stocks (autoclaved or *filter-sterilized):
NaHCO3
(*, 8%) 		30.00 ml - -
Modified trace element mixture 1.00 ml - -
Vitamin solution
(*) 		1.00 ml - -
Selenite-tungstate solution 0.50 ml - -
L-Rhamnose
(*, 0.5 M) 		10.00 ml - -
Yeast extract
(10 %) 		5.00 ml - -
2 Readjust pH to 7.2-7.4, if necessary. Aseptically and anaerobically distribute the medium into culture vessels (e.g., 10 ml in Hungate tubes) under the same gas mixture and seal with butyl rubber stoppers. Prior to use, add per liter the following solution (autoclaved and stored under N2):
Na2S x 9 H2O
(5%) 		5.00 ml - -

Modified trace element mixture #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 5.2 g 5.2 -
FeCl2 x 4 H2O 1.0 g 1 5.03
H3BO3 30.0 mg 0.03 0.485
MnCl2 x 4 H2O 100.0 mg 0.1 -
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
ZnSO4 x 7 H2O 144.0 mg 0.144 0.501
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 1000.0 ml - -
1 Dissolve EDTA x Na2 in distilled water first and adjust pH to 6.5 with NaOH. Then, proceed to add minerals.

Vitamin solution #

1000
Solution occurs in 4 other media (first defined in Medium J556).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 50 mg 0.05 0.037
p-Aminobenzoic acid 50 mg 0.05 0.365
Biotin 10 mg 0.01 0.041
Nicotinic acid 100 mg 0.1 0.812
DL-Calcium pantothenate 25 mg 0.025 0.052
Pyridoxine hydrochloride 250 mg 0.25 -
Thiamine HCl 50 mg 0.05 -
Distilled water 1000 ml - -

Selenite-tungstate solution #

1000
Solution occurs in 71 other media (first defined in Medium J431).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.4 g 0.4 10.001
Na2SeO3 x 5 H2O 6.0 mg 0.006 0.023
Na2WO4 x 2 H2O 8.0 mg 0.008 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Medium type: Complex medium
Final pH: 6.5
Equipment needed: Anaerobic workbench, Autoclave, Filter, Hungate tubes
Edit in Medium builder

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 0.992 16.976
KCl 0.496 6.654
MgCl2 x 6 H2O 0.397 1.952
NH4Cl 0.248 4.637
KH2PO4 0.198 1.458
Show more
CSV
JSON