J1305
1305 .
HELICOBACTER MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1305 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Brucella Broth
(BD-BBL) |
2.8 | g | 27.451 | - | |
| Distilled water | 80.0 | ml | - | - | |
| 1 Autoclave. After cooling to 50C and add aseptically 135 �l of conc. HCl (final conc. 0.05%). Then add aseptically the following solutions. | |||||
|
Fetal bovine serum
(Biowest; heat inactivated) |
20.0 | ml | - | - | |
|
Vitox
(Oxoid) (1 vial/10 ml of rehydration fluid) |
2.0 | ml | - | - | |
|
Skirrow supplement
(Oxoid) (1 vial/2 ml of sterile, purified water) |
0.4 | ml | - | - | |
|
Amphotericin B
(5 mg/ml DMSO) |
100.0 | �l | - | - | |
| 2 For stable culture, incubate the microorganisms in 5 ml of above-mentioned broth in a polystyrene culture flask (25 cmz2 flask, canted neck, vent cap, AS ONE: Cat. No. 2-8589-01) at 37C under a 5% O2 and 12% CO2 atmosphere (with shaking at low speed; 50 rpm). | |||||
Metadata
| Last modified: | 24.02.22 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Gas composition
- 12 % CO2
- 5 % O2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .