J1308
1308 .
MINERAL MEDIUM FOR HYDROGENOPHILUS SS56
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1308 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 2.3 | g | 2.289 | 16.817 | |
| Na2HPO4 x 2 H2O | 2.9 | g | 2.886 | 20.327 | |
| NH4Cl | 1.0 | g | 0.995 | 18.602 | |
| NaHCO3 | 0.5 | g | 0.498 | 5.922 | |
| CaCl2 x 2 H2O | 0.1 | g | 0.1 | 0.677 | |
| SL-6 trace element solution | 5.0 | ml | - | - | |
| Distilled water | 850.0 | ml | - | - | |
| 1 Mix components thoroughly, adjust pH to 6.8 and autoclave. After cooling, add the following solutions (autoclaved): | |||||
|
MgSO4 x 7 H2O
(0.5%) |
100.0 | ml | - | - | |
|
Fe(NH4)citrate
(0.1%) |
20.0 | ml | - | - | |
|
Yeast extract
(1.0%) |
10.0 | ml | - | - | |
|
Sodium lactate
(1.0 M) |
20.0 | ml | - | - | |
| 2 Aseptically distribute the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) and seal with butyl rubber stoppers. Replace the gas phase with N2 containing 5-10% O2 (by volume). | |||||
Solution occurs in 10
other media (first defined in Medium J167).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
| MnCl2 x 4 H2O | 0.03 | g | 0.03 | 0.152 | |
| H3BO3 | 0.30 | g | 0.3 | 4.851 | |
| CoCl2 x 6 H2O | 0.20 | g | 0.2 | 1.54 | |
| CuCl2 x 2 H2O | 0.01 | g | 0.01 | 0.059 | |
| NiCl2 x 6 H2O | 0.02 | g | 0.02 | 0.154 | |
| Na2MoO4 x 2 H2O | 0.03 | g | 0.03 | 0.124 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 3.6. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | 6.8 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Gas composition
- 10 % O2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .