Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Salt water
(see below, 30%) |
830.000 | ml | - | - | |
Peptone
(Oxoid) |
0.025 | g | 0.025 | - | |
Yeast extract | 5.000 | mg | 0.005 | - | |
1 Mix components and autoclave. After cooling, add the following solutions (autoclaved or *filter-sterilized): | |||||
NH4Cl
(1M) |
5.000 | ml | - | - | |
K2HPO4
(0.5 M) |
2.000 | ml | - | - | |
FeSO4 x 7 H2O
(*, 0.15%) |
5.000 | ml | - | - | |
Trace element solution | 5.000 | ml | - | - | |
Vitamin solution
(*) |
3.000 | ml | - | - | |
Sodium pyruvate
(1.0 M) |
50.000 | ml | - | - | |
Sodium perchlorate
(1.0 M) |
50.000 | ml | - | - | |
Sodium Thiosulfate
(1.0 M) |
50.000 | ml | - | - | |
2 30% Salt water: | |||||
NaCl | 240.000 | g | 238.806 | 4086.34 | |
MgCl2 x 6 H2O | 24.900 | g | 24.776 | 121.868 | |
MgSO4 x 7 H2O | 29.000 | g | 28.856 | 117.073 | |
KCl | 7.000 | g | 6.965 | 93.43 | |
CaCl2
(1M) |
5.000 | ml | - | - | |
3 Aseptically distribute the medium into culture vessels (e.g., 10 ml medium in Balch tubes), seal with butyl rubber stoppers and replace the gas phase with N2. | |||||
4 Add components to distilled water and bring volume to 1.0 L. Adjust pH to 7.5. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Thiamine HCl | 33 | mg | 0.033 | - |
Riboflavin | 10 | mg | 0.01 | 0.027 |
Nicotinic acid | 33 | mg | 0.033 | 0.268 |
DL-Calcium pantothenate | 17 | mg | 0.017 | 0.036 |
Vitamin B12 | 17 | mg | 0.017 | 0.013 |
p-Aminobenzoic acid | 13 | mg | 0.013 | 0.095 |
Distilled water | 1000 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
ZnCl2 | 70 | mg | 0.07 | 0.514 |
MnCl2 x 4 H2O | 100 | mg | 0.1 | 0.505 |
H3BO3 | 6 | mg | 0.006 | 0.097 |
CoCl2 x 6 H2O | 190 | mg | 0.19 | 1.463 |
CuCl2 x 2 H2O | 2 | mg | 0.002 | 0.012 |
NiCl2 x 6 H2O | 24 | mg | 0.024 | 0.185 |
Na2MoO4 x 2 H2O | 36 | mg | 0.036 | 0.149 |
Distilled water | 1000 | ml | - | - |
Last modified: | 22.02.22 |
Source: | JCM |
Medium type: | Complex medium |
Final pH: | 7.5 |
Equipment needed: | Autoclave, Filter |
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