J1312
1312 .
MODIFIED MV MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1312 #
ml
Equipment needed: Anaerobic workbench
Filter
Autoclave
| 1 Mix ingredients, bring to a boil and cool down under a N2-CO2 (4:1, v/v) gas stream and autoclave under the same gas mixture. After cooling, aseptically and anaerobically add per liter the following solutions (filter-sterilized): | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 1.000 | g | 8.929 | 166.92 | |
| NaCl | 2.100 | g | 18.75 | 320.842 | |
| MgCl2 x 6 H2O | 0.825 | g | 7.366 | 36.232 | |
| CaCl2 x 2 H2O | 0.100 | g | 0.893 | 6.073 | |
| KH2PO4 | 0.500 | g | 4.464 | 32.805 | |
| Trace element solution SL-10 | 1.000 | ml | - | - | |
| Selenite-tungstate solution | 1.000 | ml | - | - | |
|
Yeast extract
(BD-Difco) |
1.000 | g | 8.929 | - | |
|
Resazurin
(0.1%) |
0.500 | ml | - | - | |
| Trace vitamins | 10.000 | ml | - | - | |
|
NaHCO3
(8%) |
30.000 | ml | - | - | |
|
Taurine
(0.5 M) |
20.000 | ml | - | - | |
|
Sodium pyruvate
(1 M) |
20.000 | ml | - | - | |
|
Sodium L-lactate
(0.5 M) |
20.000 | ml | - | - | |
| Hemin solution | 10.000 | ml | - | - | |
| 2 Prior to inoculation, aseptically and anaerobically add 0.01 volume of L-cysteine x HCl x H2O to the medium (3% solution, autoclaved and stored under a N2 atmosphere). | |||||
Solution occurs in 20
other media (first defined in Medium J433).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
HCl
(25%, 7.7 M) |
10.0 | ml | 2.5 | 68.568 | |
| FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
| ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
| H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
| Distilled water | 990.0 | ml | - | - | |
| 1 Dissolve FeCl2 x 4H2O first in HCl. Then dilute in distilled water and add the remaining salts. | |||||
Solution occurs in 178
other media (first defined in Medium 385).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| NaOH | 0.5 | g | 0.5 | 12.501 |
| Na2SeO3 x 5 H2O | 3.0 | mg | 0.003 | 0.011 |
| Na2WO4 x 2 H2O | 4.0 | mg | 0.004 | 0.012 |
| Distilled water | 1000.0 | ml | - | - |
Solution occurs in 220
other media (first defined in Medium J197).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 29.10.21 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Equipment needed: | Anaerobic workbench, Autoclave, Filter |
| Edit in Medium builder | |