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JCM 1313 . MODIFIED AM5 MEDIUM 2

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1313 #

ml
Equipment needed: Autoclave Anaerobic workbench Filter
1 Mix components thoroughly and autoclave under a N2-CO2 (9:1, v/v) gas mixture. After cooling, aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized):
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 1.0 g 0.998 17.077
KCl 0.5 g 0.499 6.694
MgCl2 x 6 H2O 0.4 g 0.399 1.964
CaCl2 x 2 H2O 0.1 g 0.1 0.679
NH4Cl 0.3 g 0.299 5.597
KH2PO4 0.2 g 0.2 1.467
L-Cysteine HCl x H2O 0.2 g 0.2 -
Resazurin 0.4 mg 4.0e-4 0.002
Distilled water 910.0 ml - -
NaHCO3
(*, 8%) 		40.0 ml - -
Trace element solution 1.0 ml - -
FeCl2 solution 1.0 ml - -
Selenite-tungstate solution 1.0 ml - -
Trace vitamins (see Medium No. 197)
(*) 		10.0 ml - -
Vitamin B12 solution (see Medium No. 403)
(*) 		1.0 ml - -
Aromatic fatty acids solution 1.0 ml - -
Menadione solution
(see below) 		1.0 ml - -
Fatty acid mixture (see Medium No. 266)
(*) 		1.0 ml - -
Yeast extract
(*, 10%) 		10.0 ml - -
Casamino acids
(*, 10%) 		10.0 ml - -
Glucose
(1 M) 		5.0 ml - -
Dithiothreitol
(*, 0.1 M) 		10.0 ml - -
2 Readjust pH to 7.3, if necessary.
3 Menadione solution:
4 Autoclave 100 mg menadione (vitamin K3) with 100 ml 5% NaHCO3 solution. Remove insoluble residue by filtration. Store the solution under a N2-CO2 (8:2, v/v) gas mixture.

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

FeCl2 solution #

1000
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -

Selenite-tungstate solution #

1000
Solution occurs in 71 other media (first defined in Medium J431).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.4 g 0.4 10.001
Na2SeO3 x 5 H2O 6.0 mg 0.006 0.023
Na2WO4 x 2 H2O 8.0 mg 0.008 0.024
Distilled water 1000.0 ml - -

Aromatic fatty acids solution #

100
Solution occurs in 1 other media (first defined in Medium J1051).
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Phenyl acetic acid 68 mg 0.68 4.994
Phenyl propionic acid 75 mg 0.75 4.994
4-Hydroxyphenyl acetic acid 76 mg 0.76 4.995
3-Indolyl acetic acid 88 mg 0.88 5.023
NaOH
(20 mM) 		100 ml - -
1 Autoclave and store under a N2 atmosphere.

Metadata

Last modified: 22.02.22
Source: JCM
Medium type: Complex medium
Final pH: 7.3
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 0.998 17.077
KCl 0.499 6.694
MgCl2 x 6 H2O 0.399 1.964
NH4Cl 0.299 5.597
KH2PO4 0.2 1.467
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