J1320
1320 .
THERMODESULFOVIBRIO MEDIUM FOR N1
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1320 #
ml
Equipment needed: Autoclave
Hungate tubes
Anaerobic workbench
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 1.00 | g | 0.941 | 16.097 | |
| MgCl2 x 6 H2O | 0.40 | g | 0.376 | 1.851 | |
| CaCl2 x 2 H2O | 0.15 | g | 0.141 | 0.96 | |
| NH4Cl | 0.25 | g | 0.235 | 4.397 | |
| NaH2PO4 | 0.20 | g | 0.188 | 1.568 | |
| KCl | 0.50 | g | 0.47 | 6.309 | |
| Na2SO4 | 4.00 | g | 3.763 | 26.492 | |
| Wolfe's mineral solution | 10.00 | ml | - | - | |
| Yeast extract | 1.00 | g | 0.941 | - | |
| Resazurin | 0.50 | mg | 4.7e-4 | 0.002 | |
|
Iron metal
(powder) |
10.00 | g | 9.407 | 168.454 | |
| Distilled water | 988.00 | ml | - | - | |
| 1 Mix components thoroughly, except iron metal, and autoclave under a N2 gas atmosphere. After cooling, add the following solutions from sterile anaerobic stocks (autoclaved or *filter-sterilized): | |||||
|
NaHCO3
(*, 8%) |
15.00 | ml | - | - | |
|
L-Sodium lactate
(1.0 M) |
20.00 | ml | - | - | |
| Trace vitamins | 10.00 | ml | - | - | |
| 2 Separately autoclave and dry iron metal (powder). Aseptically distribute the medium and iron metal into culture vessels (e.g., 10 ml medium and 100 mg iron in Hungate tubes) under a N2 gas stream and seal with butyl rubber stoppers. Prior to use, aseptically and anaerobically add per liter the following solution (filter-sterilized): | |||||
|
Sodium thioglycolate
(2%) |
10.00 | ml | - | - | |
|
L-Ascorbic acid
(2%) |
10.00 | ml | - | - | |
| 3 Adjust pH to 8.0, if necessary. | |||||
Solution occurs in 7
other media (first defined in Medium J265).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Trace minerals | 1000.00 | ml | - | - |
| NiCl2 x 6 H2O | 0.02 | g | 0.02 | 0.154 |
| Na2SeO3 | 1.00 | mg | 1.0e-3 | - |
| Na2WO4 x 2 H2O | 0.01 | g | 0.01 | 0.03 |
Solution occurs in 220
other media (first defined in Medium J197).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Solution occurs in 97
other media (first defined in Medium J151).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
| MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
| MnSO4 x n H2O | 0.50 | g | 0.5 | - | |
| NaCl | 1.00 | g | 1 | 17.112 | |
| FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
| CoSO4 x 7 H2O | 0.10 | g | 0.1 | 0.645 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
| CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
| AlK(SO4)2 | 0.01 | g | 0.01 | 0.039 | |
| H3BO3 | 0.01 | g | 0.01 | 0.162 | |
| Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | 8.0 |
| Equipment needed: | Anaerobic workbench, Autoclave, Filter, Hungate tubes |
| Edit in Medium builder | |