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JCM 1326 . MODIFIED DESULFOVIBRIO MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1326 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 0.5 g 0.494 2.837
KH2PO4 0.5 g 0.494 3.631
NH4Cl 1.0 g 0.988 -
Na2SO4 1.0 g 0.988 6.957
MgSO4 x 7 H2O 2.0 g 1.976 8.018
CaCl2 x 2 H2O 0.1 g 0.099 0.672
Sodium lactate 2.0 g 1.976 17.636
Yeast extract 1.0 g 0.988 -
FeCl2 solution 1.0 ml - -
Trace element solution 1.0 ml - -
Trace vitamins 10.0 ml - -
Resazurin 1.0 mg 9.9e-4 0.004
Distilled water 980.0 ml - -
1 SolutionB:
FeSO4 x 7 H2O 0.1 g 0.099 0.355
Distilled water 10.0 ml - -
2 SolutionC:
Sodium thioglycolate 0.1 g 0.099 0.866
Ascorbic acid 0.1 g 0.099 0.561
Distilled water 10.0 ml - -
3 Solution A:
4 Dissolve ingredients of each solution in the appropriate amount of distilled water. Bring solution A to a boil and cool down to room temperature under a N2 gas stream. Add solutions Band C to the medium, then, adjust pH to 7.3 with NaOH. While swirling the medium, dispense into culture vessels under a N2 gas stream, seal with butyl rubber stoppers and autoclave.

FeCl2 solution #

1000
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Medium type: Complex medium
Final pH: 7.3
Equipment needed: Autoclave
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Sodium lactate 1.976 17.636
MgSO4 x 7 H2O 1.976 8.018
Na2SO4 0.988 6.957
NH4Cl 0.988 -
Yeast extract 0.988 -
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