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J1326. MODIFIED DESULFOVIBRIO MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.494 2.837
2 0.5 g 0.494 3.631
3 1.0 g 0.988 -
4 1.0 g 0.988 6.957
5 2.0 g 1.976 8.018
6 0.1 g 0.099 0.672
7 2.0 g 1.976 -
8 1.0 g 0.988 -
9 FeCl2 solution 1.0 ml - -
10 Trace element solution 1.0 ml - -
11 Trace vitamins 10.0 ml - -
12 1.0 mg 9.9e-4 0.004
13 980.0 ml 966.643 53656.8
14 0.1 g 0.099 0.355
15 10.0 ml 9.864 547.518
16 0.1 g 0.099 -
17 0.1 g 0.099 -
18 10.0 ml 9.864 547.518
Instructions:
  1. Solution A:
  2. SolutionB: #14-15
  3. SolutionC: #16-18
  4. Dissolve ingredients of each solution in the appropriate amount of distilled water. Bring solution A to a boil and cool down to room temperature under a N2 gas stream. Add solutions Band C to the medium, then, adjust pH to 7.3 with NaOH. While swirling the medium, dispense into culture vessels under a N2 gas stream, seal with butyl rubber stoppers and autoclave.
Solution occurs in 89 other media (first defined in medium J187 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 70 mg 0.07 0.514
2 100 mg 0.1 -
3 6 mg 0.006 0.097
4 190 mg 0.19 -
5 2 mg 0.002 -
6 24 mg 0.024 -
7 36 mg 0.036 0.149
8 1000 ml 998.207 55408.8
Solution occurs in 87 other media (first defined in medium J187 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(7.7 M, 25%)
10.0 ml 3.17 86.943
2 1.5 g 1.5 -
3 990.0 ml 988.225 54854.8
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.197 55408.3
MgSO4 x 7H2O 1.976 8.018
Sodium lactate 1.976 -
Na2SO4 0.988 6.957
NH4CI 0.988 -
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