Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 18.00 | g | 17.613 | 301.378 | |
MgCl2 x 6 H2O | 2.60 | g | 2.544 | 12.514 | |
CaCl2 x 2 H2O | 0.15 | g | 0.147 | 0.998 | |
NH4Cl | 0.25 | g | 0.245 | 4.573 | |
KH2PO4 | 0.20 | g | 0.196 | 1.438 | |
KCl | 0.50 | g | 0.489 | 6.563 | |
NaNO3 | 0.85 | g | 0.832 | 9.785 | |
Glucose | 1.80 | g | 1.761 | 9.776 | |
Yeast extract
(BD-Difco) |
1.00 | g | 0.978 | - | |
Trace minerals
(see Medium No. 243) |
1.00 | ml | - | - | |
Trace vitamins | 1.00 | ml | - | - | |
NaHCO3 | 2.52 | g | 2.466 | 29.352 | |
Distilled water | 1000.00 | ml | - | - | |
1 Mix components, and adjust pH to 7.0. Dispense the medium into suitable culture vessels under a stream of N2-CO2 (80:20, v/v), seal with butyl rubber stoppers, and autoclave. Prior to inoculation, aseptically and anaerobically add per liter the following solutions (autoclaved and stored under a N2 atmosphere): | |||||
Na2S x 9 H2O
(5%) |
10.00 | ml | - | - | |
L-Cysteine HCl x H2O
(5%) |
10.00 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 2.0 | mg | 0.002 | 0.008 |
Folic acid | 2.0 | mg | 0.002 | 0.005 |
Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
Riboflavin | 5.0 | mg | 0.005 | 0.013 |
Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Last modified: | 22.02.22 |
Source: | JCM |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 7.0 |
Equipment needed: | Anaerobic workbench, Autoclave |
Edit in Medium builder |
All strains for this medium: | 3 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 17.613 | 301.378 |
MgCl2 x 6 H2O | 2.544 | 12.514 |
NaHCO3 | 2.466 | 29.352 |
Glucose | 1.761 | 9.776 |
Yeast extract | 0.978 | - |
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