14 .
EG MEDIUM
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Equipment needed:
Final pH:
7.7
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Lab-Lemco beef extract
(Oxoid) |
2.4 | g | ||
|
|
Proteose peptone no. 3
(BD-Difco) |
10.0 | g | ||
|
|
Yeast extract
(BD-Difco) |
5.0 | g | ||
|
|
Na2HPO4 | 4.0 | g | ||
|
|
Glucose | 1.5 | g | ||
|
|
Starch
(soluble) |
0.5 | g | ||
|
|
L-Cystine | 0.2 | g | ||
|
|
Agar | 15.0 | g | ||
|
|
L-Cysteine HCl x H2O | 0.5 | g | ||
|
|
Horse blood | 50.0 | ml | ||
|
|
Distilled water | 950.0 | ml | ||
| 1 Add L-cystine to 50 ml of 1 N HCl and mix thoroughly. Add the remaining components and bring volume to 950 ml. Adjust pH to 7.6-7.8. After autoclaving and then cooling to 50C, aseptically add 50.0 ml of horse blood. Mix thoroughly and pour into sterile petri dishes or distribute into sterile tubes. | |||||