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JCM 151 . PYROCOCCUS MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J151 #

ml
Equipment needed: Anaerobic workbench Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Salt base solution 1000.0 ml - -
Trace minerals 10.0 ml - -
Citric acid 5.0 mg 0.005 0.026
Yeast extract
(BD-Difco) 		1.0 g 0.99 -
Bacto peptone
(BD-Difco) 		5.0 g 4.951 -
Sulfur
(powder) 		30.0 g 29.703 926.192
Na2S x 9 H2O 0.5 g 0.495 2.061
Resazurin 1.0 mg 9.9e-4 0.004
1 Mix ingredients, except sulfur and Na2S x 9H2O, and adjust pH to 6.5 with H2SO4. Sterilize by filtration under a N2 atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Neutralize Na2S x 9H2O as a 5% solution, and autoclave under a N2 atmosphere. Aseptically and anaerobically add sterile sulfur to the medium. Prior to inoculation, add the sterile Na2S x 9H2O solution, and readjust pH to 6.5, if necessary.

Salt base solution #

1000
Solution occurs in 1 other media (first defined in Medium J151).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 13.850 g 13.85 236.995
MgSO4 x 7 H2O 3.500 g 3.5 14.2
MgCl2 x 6 H2O 2.750 g 2.75 13.527
KCl 0.325 g 0.325 4.359
NaBr 0.050 g 0.05 0.486
H3BO3 15.000 mg 0.015 0.243
SrCl2 x 6 H2O 7.500 mg 0.008 0.028
KI 0.050 mg 5.0e-5 1.9e-4
CaCl2 0.750 g 0.75 6.758
KH2PO4 0.500 g 0.5 3.674
(NH4)2Ni(SO4)2 x 6 H2O 2.000 mg 0.002 0.005
Distilled water 1000.000 ml - -

Trace minerals #

1000
Solution occurs in 97 other media (first defined in Medium J151).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x n H2O 0.50 g 0.5 -
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.10 g 0.1 0.645
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 0.01 g 0.01 0.039
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 6.5
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 6

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Sulfur 29.703 926.192
NaCl 13.723 234.818
Bacto peptone 4.951 -
MgSO4 x 7 H2O 3.495 14.18
MgCl2 x 6 H2O 2.723 13.393
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Cultivation metadata from 6 strains BacDive

phyla 2 6 strains
oxygen anaerobe 5 strains
temperature hyperthermophilic 6 strains