Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Salt base solution | 1000.0 | ml | - | - | |
Trace minerals | 10.0 | ml | - | - | |
Citric acid | 5.0 | mg | 0.005 | 0.026 | |
Yeast extract
(BD-Difco) |
1.0 | g | 0.99 | - | |
Bacto peptone
(BD-Difco) |
5.0 | g | 4.951 | - | |
Sulfur
(powder) |
30.0 | g | 29.703 | 926.192 | |
Na2S x 9 H2O | 0.5 | g | 0.495 | 2.061 | |
Resazurin | 1.0 | mg | 9.9e-4 | 0.004 | |
1 Mix ingredients, except sulfur and Na2S x 9H2O, and adjust pH to 6.5 with H2SO4. Sterilize by filtration under a N2 atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Neutralize Na2S x 9H2O as a 5% solution, and autoclave under a N2 atmosphere. Aseptically and anaerobically add sterile sulfur to the medium. Prior to inoculation, add the sterile Na2S x 9H2O solution, and readjust pH to 6.5, if necessary. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
NaCl | 13.850 | g | 13.85 | 236.995 |
MgSO4 x 7 H2O | 3.500 | g | 3.5 | 14.2 |
MgCl2 x 6 H2O | 2.750 | g | 2.75 | 13.527 |
KCl | 0.325 | g | 0.325 | 4.359 |
NaBr | 0.050 | g | 0.05 | 0.486 |
H3BO3 | 15.000 | mg | 0.015 | 0.243 |
SrCl2 x 6 H2O | 7.500 | mg | 0.008 | 0.028 |
KI | 0.050 | mg | 5.0e-5 | 1.9e-4 |
CaCl2 | 0.750 | g | 0.75 | 6.758 |
KH2PO4 | 0.500 | g | 0.5 | 3.674 |
(NH4)2Ni(SO4)2 x 6 H2O | 2.000 | mg | 0.002 | 0.005 |
Distilled water | 1000.000 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
MnSO4 x n H2O | 0.50 | g | 0.5 | - | |
NaCl | 1.00 | g | 1 | 17.112 | |
FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
CoSO4 x 7 H2O | 0.10 | g | 0.1 | 0.645 | |
CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
AlK(SO4)2 | 0.01 | g | 0.01 | 0.039 | |
H3BO3 | 0.01 | g | 0.01 | 0.162 | |
Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0. |
Last modified: | 22.02.22 |
Source: | JCM |
Taxonomic range: | Archaea |
Medium type: | Complex medium |
Final pH: | 6.5 |
Equipment needed: | Anaerobic workbench, Autoclave, Filter |
Edit in Medium builder |
All strains for this medium: | 6 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Sulfur | 29.703 | 926.192 |
NaCl | 13.723 | 234.818 |
Bacto peptone | 4.951 | - |
MgSO4 x 7 H2O | 3.495 | 14.18 |
MgCl2 x 6 H2O | 2.723 | 13.393 |
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