J180
180 .
THERMOPLASMA MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J180 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| (NH4)2SO4 | 0.20 | g | 0.2 | 1.514 | |
| KH2PO4 | 3.00 | g | 3 | 22.045 | |
| MgSO4 x 7 H2O | 0.50 | g | 0.5 | 2.029 | |
| CaCl2 x 2 H2O | 0.25 | g | 0.25 | 1.701 | |
|
Yeast extract
(BD-Difco) |
1.00 | g | 1 | - | |
| Glucose | 10.00 | g | 10 | 55.506 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 2.0 with 10 N H2SO4. Separately autoclave yeast extract and glucose as 10% solutions, and add aseptically to the medium. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Taxonomic range: | Archaea |
| Medium type: | Complex medium |
| Final pH: | 2.0 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 4 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Glucose | 10 | 55.506 |
| KH2PO4 | 3 | 22.045 |
| Yeast extract | 1 | - |
| MgSO4 x 7 H2O | 0.5 | 2.029 |
| CaCl2 x 2 H2O | 0.25 | 1.701 |
| Show more | ||
