Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Modified Brock's salt base solution | 1000.0 | ml | - | - | |
Yeast extract
(BD-Difco) |
1.0 | g | 1 | - | |
Sulfur
(powder) |
5.0 | g | 5 | 155.909 | |
Na2S x 9 H2O | 0.5 | g | 0.5 | 2.082 | |
Resazurin | 1.0 | mg | 1.0e-3 | 0.004 | |
1 Mix ingredients, except yeast extract, sulfur and Na2S x 9H2O, and autoclave under a N2 atmosphere. Separately autoclave yeast extract as a 10% solution and Na2S x 9H2O as a 5% solution under a N2 atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Aseptically and anaerobically add the yeast extract solution and sulfur to the medium. Prior to inoculation, add the sterile Na2S x 9H2O solution, and adjust pH to 5.5 with sterile 1 N H2SO4. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
(NH4)2SO4 | 1.30 | g | 1.3 | 9.838 |
KH2PO4 | 0.28 | g | 0.28 | 2.058 |
MgSO4 x 7 H2O | 0.25 | g | 0.25 | 1.014 |
CaCl2 x 2 H2O | 0.07 | g | 0.07 | 0.476 |
FeCl3 x 6 H2O | 2.00 | mg | 0.002 | 0.007 |
MnCl2 x 4 H2O | 1.80 | mg | 0.002 | 0.009 |
Na2B4O7 x 10 H2O | 4.50 | mg | 0.005 | - |
ZnSO4 x 7 H2O | 0.22 | mg | 2.2e-4 | 7.7e-4 |
CuCl2 x 2 H2O | 0.05 | mg | 5.0e-5 | 2.9e-4 |
Na2MoO4 x 2 H2O | 0.03 | mg | 3.0e-5 | 1.2e-4 |
VOSO4 x n H2O | 0.03 | mg | 3.0e-5 | - |
CoSO4 x 7 H2O | 0.01 | mg | 1.0e-5 | 6.5e-5 |
Distilled water | 1000.00 | ml | - | - |
Last modified: | 22.02.22 |
Source: | JCM |
Taxonomic range: | Archaea |
Medium type: | Complex medium |
Final pH: | 5.5 |
Equipment needed: | Anaerobic workbench, Autoclave |
Edit in Medium builder |
All strains for this medium: | 2 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Sulfur | 5 | 155.909 |
(NH4)2SO4 | 1.3 | 9.838 |
Yeast extract | 1 | - |
Na2S x 9 H2O | 0.5 | 2.082 |
KH2PO4 | 0.28 | 2.058 |
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