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JCM 187 . DESULFUROCOCCUS AMYLOLYTICUS MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Anaerobic workbench Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.33 g 0.329 6.157
KH2PO4 0.33 g 0.329 2.42
KCl 0.33 g 0.329 4.418
CaCl2 x 2 H2O 0.44 g 0.439 2.987
MgCl2 x 6 H2O 0.70 g 0.699 3.436
NaCl 0.50 g 0.499 8.539
NaHCO3 0.80 g 0.798 9.504
FeCl2 solution 1.00 ml - -
Trace element solution 1.00 ml - -
Trace vitamins 10.00 ml - -
Yeast extract
(BD-Difco)
0.20 g 0.2 -
Bacto peptone
(BD-Difco)
5.00 g 4.99 -
Sulfur
(powder)
10.00 g 9.98 311.195
Na2S x 9 H2O 0.50 g 0.499 2.078
Resazurin 1.00 mg 1.0e-3 0.004
Distilled water 990.00 ml - -
1 Mix ingredients, except yeast extract, peptone, vitamins, sulfur and Na2S x 9H2O, and autoclave under a N2-CO2 (80:20) atmosphere. Separately autoclave yeast extract and peptone as 10% solutions and Na2S x 9H2O as a 5% solution under a N2 atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Filter-sterilize NaHCO3 as an 8% solution and vitamin solution while gassing with N2. Aseptically and anaerobically add yeast extract, peptone, NaHCO3 and vitamin solutions, and sulfur to the medium under a N2-CO2 (80:20) atmosphere. Prior to inoculation, add the sterile Na2S x 9H2O solution, and adjust pH to 6.2-6.4 with sterile 1 N H2SO4.
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%)
10.0 ml - -
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 -
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 -
Thiamine HCl 5.0 mg 0.005 -
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 6.3
Equipment needed: Anaerobic workbench, Autoclave, Filter

Associated strains

All strains for this medium: 6

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Sulfur 9.98 311.195
Bacto peptone 4.99 -
NaHCO3 0.798 9.504
MgCl2 x 6 H2O 0.699 3.436
NaCl 0.499 8.539
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Cultivation metadata from 6 strains BacDive

pH 4 - 6 3 strains
pH 6 - 8 2 strains
phyla 1 5 strains
nutrition chemoorganoheterotroph 2 strains
oxygen anaerobe 4 strains
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