Name: THERMOPROTEUS TENAX MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. J196 #

Equipment needed: Autoclave Anaerobic workbench Filter

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
(NH₄)₂SO₄	0.264	g	0.264	1.998
FeSO₄ x 7 H₂O	0.556	g	0.556	2
MgSO₄ x 7 H₂O	0.492	g	0.492	1.996
CaSO₄ x 2 H₂O	0.344	g	0.344	1.998
KH₂PO₃	0.014	g	0.014	-
NaF	0.840	mg	8.4e-4	0.02
MnCl₂ x 4 H₂O	0.180	mg	1.8e-4	-
Na₂B₄O₇ x 10 H₂O	0.450	mg	4.5e-4	-
ZnSO₄ x 7 H₂O	22.000	ï¿½g	-	-
CuCl₂ x 2 H₂O	5.000	ï¿½g	-	-
Na₂MoO₇ x 2 H₂O	3.000	ï¿½g	-	-
CoSO₄ x 7 H₂O	1.000	ï¿½g	-	-
Yeast extract (BD-Difco)	0.200	g	0.2	-
Starch (soluble)	5.000	g	5	-
Sulfur (powder)	10.000	g	10	311.818
Na₂S x 9 H₂O	0.500	g	0.5	2.082
Resazurin	1.000	mg	1.0e-3	0.004
Distilled water	1000.000	ml	-	-
1 Mix ingredients, except yeast extract, starch, sulfur and Na₂S x 9H2O, and filter-sterilize while gassing with N₂. Autoclave yeast extract and starch as 10% solutions and Na₂S x 9H2O as a 5% solution under a N₂ atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Aseptically and anaerobically add the yeast extract and starch solutions, and sulfur to the medium. Prior to inoculation, add the sterile Na₂S x 9H2O solution, and adjust pH to 5.5 with sterile 1 N H₂SO₄.

Compound

Conc. [g/L]

Conc. [mM]

Sulfur

311.818

Starch

FeSO₄ x 7 H₂O

0.556

Na₂S x 9 H₂O

0.5

2.082

MgSO₄ x 7 H₂O

0.492

1.996

Last modified:	22.02.22
Source:	JCM
Taxonomic range:	Archaea
Medium type:	Complex medium
Final pH:	5.5
Equipment needed:	Anaerobic workbench, Autoclave, Filter

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J196
196 . THERMOPROTEUS TENAX MEDIUM