196 .
THERMOPROTEUS TENAX MEDIUM
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Equipment needed:
Anaerobic workbench
Autoclave
Filter
Final pH:
5.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
(NH4)2SO4 | 0.264 | g | ||
|
|
FeSO4 x 7 H2O | 0.556 | g | ||
|
|
MgSO4 x 7 H2O | 0.492 | g | ||
|
|
CaSO4 x 2 H2O | 0.344 | g | ||
|
|
KH2PO3 | 0.014 | g | ||
|
|
NaF | 0.840 | mg | ||
|
|
MnCl2 x 4 H2O | 0.180 | mg | ||
|
|
Na2B4O7 x 10 H2O | 0.450 | mg | ||
|
|
ZnSO4 x 7 H2O | 22.000 | �g | ||
|
|
CuCl2 x 2 H2O | 5.000 | �g | ||
|
|
Na2MoO7 x 2 H2O | 3.000 | �g | ||
|
|
CoSO4 x 7 H2O | 1.000 | �g | ||
|
|
Yeast extract
(BD-Difco) |
0.200 | g | ||
|
|
Starch
(soluble) |
5.000 | g | ||
|
|
Sulfur
(powder) |
10.000 | g | ||
|
|
Na2S x 9 H2O | 0.500 | g | ||
|
|
Resazurin | 1.000 | mg | ||
|
|
Distilled water | 1000.000 | ml | ||
| 1 Mix ingredients, except yeast extract, starch, sulfur and Na2S x 9H2O, and filter-sterilize while gassing with N2. Autoclave yeast extract and starch as 10% solutions and Na2S x 9H2O as a 5% solution under a N2 atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Aseptically and anaerobically add the yeast extract and starch solutions, and sulfur to the medium. Prior to inoculation, add the sterile Na2S x 9H2O solution, and adjust pH to 5.5 with sterile 1 N H2SO4. | |||||