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JCM 198 . JCM MEDIUM No. 198

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J198 #

ml
Equipment needed: Anaerobic workbench Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.75 g 0.736 5.408
K2HPO4 0.75 g 0.736 4.226
MnCl2 x 4 H2O 0.36 g 0.353 -
NH4Cl 1.00 g 0.981 18.347
NaHCO3 4.00 g 3.925 46.727
Trace mineral solution 9.00 ml - -
Trace vitamins 10.00 ml - -
Na2S x 9 H2O 0.50 g 0.491 2.043
L-Cysteine HCl x H2O 0.50 g 0.491 -
Resazurin 1.00 mg 9.8e-4 0.004
Distilled water 1000.00 ml - -
1 Mix ingredients, except NaHCO3, Trace vitamins, Na2S x 9H2O and L-cysteine x HCl x H2O, and autoclave under a H2-CO2 (80:20) atmosphere. Filter-sterilize NaHCO3 as 8% solution and Trace vitamins while gassing with N2. Separately autoclave Na2S x 9H2O and L-cysteine x HCl x H2O as 5% solutions under a N2 atmosphere. Aseptically and anaerobically add the NaHCO3 solution and Trace vitamins to the medium under a H2-CO2 (80:20) atmosphere. Prior to inoculation, add sterile the Na2S x 9H2O and L-cysteine x HCl solutions, and adjust pH to 7.4. Pressurize inoculated bottles to 200 kPa H2-CO2 (80:20).

Trace mineral solution #

1000
Solution occurs in 2 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 4.500 g 4.5 23.543
FeCl2 x 4 H2O 0.400 g 0.4 2.012
MnCl2 x 4 H2O 0.100 g 0.1 -
CoCl2 x 6 H2O 0.170 g 0.17 1.309
ZnCl2 0.100 g 0.1 0.734
CaCl2 x 2 H2O 0.020 g 0.02 0.136
H3BO3 0.019 g 0.019 0.307
Na2MoO4 x 2 H2O 0.010 g 0.01 0.041
NiCl2 x 6 H2O 0.030 g 0.03 0.231
Distilled water 1000.000 ml - -
1 Adjust pH to 7.0 with 1 N KOH.

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Archaea
Medium type: Defined medium
Final pH: 7.4
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 8

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 3.925 46.727
NH4Cl 0.981 18.347
KH2PO4 0.736 5.408
K2HPO4 0.736 4.226
Na2S x 9 H2O 0.491 2.043
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Cultivation metadata from 8 strains BacDive

phyla 1 8 strains
oxygen anaerobe 4 strains
temperature mesophilic 6 strains
temperature thermophilic 2 strains