JCM 198 . JCM MEDIUM No. 198

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×
Equipment needed: Anaerobic workbench Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.75 g 0.736 5.408
K2HPO4 0.75 g 0.736 4.226
MnCl2 x 4 H2O 0.36 g 0.353 -
NH4Cl 1.00 g 0.981 18.347
NaHCO3 4.00 g 3.925 46.727
Trace mineral solution 9.00 ml - -
Trace vitamins 10.00 ml - -
Na2S x 9 H2O 0.50 g 0.491 2.043
L-Cysteine HCl x H2O 0.50 g 0.491 -
Resazurin 1.00 mg 9.8e-4 0.004
Distilled water 1000.00 ml - -
1 Mix ingredients, except NaHCO3, Trace vitamins, Na2S x 9H2O and L-cysteine x HCl x H2O, and autoclave under a H2-CO2 (80:20) atmosphere. Filter-sterilize NaHCO3 as 8% solution and Trace vitamins while gassing with N2. Separately autoclave Na2S x 9H2O and L-cysteine x HCl x H2O as 5% solutions under a N2 atmosphere. Aseptically and anaerobically add the NaHCO3 solution and Trace vitamins to the medium under a H2-CO2 (80:20) atmosphere. Prior to inoculation, add sterile the Na2S x 9H2O and L-cysteine x HCl solutions, and adjust pH to 7.4. Pressurize inoculated bottles to 200 kPa H2-CO2 (80:20).
Solution occurs in 2 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 4.500 g 4.5 23.543
FeCl2 x 4 H2O 0.400 g 0.4 2.012
MnCl2 x 4 H2O 0.100 g 0.1 -
CoCl2 x 6 H2O 0.170 g 0.17 1.309
ZnCl2 0.100 g 0.1 0.734
CaCl2 x 2 H2O 0.020 g 0.02 0.136
H3BO3 0.019 g 0.019 0.307
Na2MoO4 x 2 H2O 0.010 g 0.01 0.041
NiCl2 x 6 H2O 0.030 g 0.03 0.231
Distilled water 1000.000 ml - -
1 Adjust pH to 7.0 with 1 N KOH.
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 -
Thiamine HCl 5.0 mg 0.005 -
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Archaea
Medium type: Defined medium
Final pH: 7.4
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 8

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 3.925 46.727
NH4Cl 0.981 18.347
KH2PO4 0.736 5.408
K2HPO4 0.736 4.226
Na2S x 9 H2O 0.491 2.043
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Cultivation metadata from 8 strains BacDive

phyla 1 8 strains
oxygen anaerobe 4 strains
temperature mesophilic 6 strains
temperature thermophilic 2 strains