J201
201 .
BSK MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J201 #
ml
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
CMRL 1066
(Gibco 330-1540) |
100.0 | ml | - | - | |
|
Neopeptone
(BD-Difco) |
5.0 | g | 5 | - | |
| Bovine albumin , Fraction V | 50.0 | g | 50 | - | |
| HEPES | 6.0 | g | 6 | 25.177 | |
| Trisodium citrate | 0.7 | g | 0.7 | 2.712 | |
| Glucose | 5.0 | g | 5 | 27.753 | |
| Sodium pyruvate | 0.8 | g | 0.8 | 7.27 | |
| N-Acetyl-D-glucosamine | 0.4 | g | 0.4 | 1.808 | |
| NaHCO3 | 2.2 | g | 2.2 | 26.188 | |
| Distilled water | 900.0 | ml | - | - | |
| 1 Mix all ingredients in a beaker with a stir bar. Adjust pH to 7.6. Add 200 ml of 7% gelatin. Filter-sterilize entire medium. Add sterile rabbit serum to final concentration of 6%. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | 7.6 |
| Equipment needed: | Filter |
| Edit in Medium builder | |