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JCM 203 . PYRODICTIUM BROCKII MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J203 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Salt base solution 1000 ml - -
Trace minerals 10 ml - -
Citric acid 5 mg 0.005 0.026
Yeast extract
(BD-Difco) 		2 g 1.961 -
Sulfur
(powder) 		30 g 29.412 917.111
Resazurin 1 mg 9.8e-4 0.004
1 Mix components except sulfur, adjust pH to 5.5 with H2SO4 and autoclave under a N2 gas atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Aseptically distribute the medium and sulfur into culture vessels (e.g., 20 ml of the medium and 0.6 g sulfur in 120 ml serum bottles) under a stream of a H2-CO4 (80:20, v/v) gas mixture and seal with butyl rubber stoppers. Prior to inoculation, add per litter the following solution (autoclave and stored under a N2 atmosphere):
Na2S x 9 H2O
(pH 7.0, 5%) 		10 ml - -
2 Readjust pH to 5.5, if necessary. Pressurize inoculated vessels to 200kPa H2 -CO2 (80:20, v/v).

Salt base solution #

1000
Solution occurs in 1 other media (first defined in Medium J151).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 13.850 g 13.85 236.995
MgSO4 x 7 H2O 3.500 g 3.5 14.2
MgCl2 x 6 H2O 2.750 g 2.75 13.527
KCl 0.325 g 0.325 4.359
NaBr 0.050 g 0.05 0.486
H3BO3 15.000 mg 0.015 0.243
SrCl2 x 6 H2O 7.500 mg 0.008 0.028
KI 0.050 mg 5.0e-5 1.9e-4
CaCl2 0.750 g 0.75 6.758
KH2PO4 0.500 g 0.5 3.674
(NH4)2Ni(SO4)2 x 6 H2O 2.000 mg 0.002 0.005
Distilled water 1000.000 ml - -

Trace minerals #

1000
Solution occurs in 97 other media (first defined in Medium J151).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x n H2O 0.50 g 0.5 -
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.10 g 0.1 0.645
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 0.01 g 0.01 0.039
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 5.5
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Sulfur 29.412 917.111
NaCl 13.588 232.516
MgSO4 x 7 H2O 3.461 14.041
MgCl2 x 6 H2O 2.696 13.261
Yeast extract 1.961 -
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature hyperthermophilic 1 strain