J209
209 .
M98-5
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J209 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Rumen fluid | 400.00 | ml | - | - | |
|
Trypticase peptone
(BD-BBL) |
2.00 | g | 1.832 | - | |
|
Yeast extract
(BD-Difco) |
0.50 | g | 0.458 | - | |
| Glucose | 0.30 | g | 0.275 | 1.525 | |
| Cellobiose | 0.50 | g | 0.458 | 1.338 | |
| Maltose | 0.30 | g | 0.275 | 0.803 | |
| Glycerol | 0.30 | g | 0.275 | 2.983 | |
|
Salts solution
(see below) |
50.00 | ml | - | - | |
|
Agar
(BD-Difco, Bacto ) |
20.00 | g | 18.315 | - | |
|
Hemin
(1%) |
1.00 | ml | - | - | |
|
Resazurin
(0.05%) |
0.50 | ml | - | - | |
|
Na2CO3-CO2 buffer
(4%) |
100.00 | ml | - | - | |
|
L-Ascorbic acid
(25%) |
1.00 | ml | - | - | |
|
L-Cysteine HCl x H2O
(5%) |
10.00 | ml | - | - | |
| Distilled water | 430.00 | ml | - | - | |
| 1 Salts solution | |||||
| K2HPO4 | 0.78 | g | 0.714 | 4.101 | |
| KH2PO4 | 0.47 | g | 0.43 | 3.163 | |
| NaCl | 1.18 | g | 1.081 | 18.491 | |
| (NH4)2SO4 | 1.20 | g | 1.099 | 8.316 | |
| CaCl2 x 2 H2O | 0.12 | g | 0.11 | 0.747 | |
| MgSO4 x 7 H2O | 0.25 | g | 0.229 | 0.929 | |
| Distilled water | 100.00 | ml | - | - | |
| 2 Adjust pH to 6.7-6.8. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | 6.8 |
| Edit in Medium builder | |