Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KCl | 0.34 | g | 0.34 | 4.561 | |
MgCl2 x 6 H2O | 4.00 | g | 4 | 19.675 | |
MgSO4 x 7 H2O | 3.45 | g | 3.45 | 13.997 | |
NH4Cl | 0.25 | g | 0.25 | 4.674 | |
CaCl2 x 2 H2O | 0.14 | g | 0.14 | 0.952 | |
K2HPO4 | 0.14 | g | 0.14 | 0.804 | |
NaCl | 18.00 | g | 18 | 308.008 | |
NaHCO3 | 5.00 | g | 5 | 59.519 | |
Fe(NH4)2(SO4)2 x 6 H2O | 2.00 | mg | 0.002 | - | |
(NH4)2Ni(SO4)2 x 6 H2O | 2.00 | mg | 0.002 | 0.005 | |
Trace minerals | 10.00 | ml | - | - | |
Yeast extract
(BD-Difco) |
0.50 | g | 0.5 | - | |
Sodium L-lactate | 1.50 | g | 1.5 | 13.386 | |
Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
Na2S x 9 H2O | 0.50 | g | 0.5 | 2.082 | |
Distilled water | 990.00 | ml | - | - | |
1 Mix ingredients, except Na2S x 9H2O, and adjust pH to 6.9 with 1 N H2SO4. Sterilize by filtration under a N2-CO2 (80:20) gas mixture. Neutralize Na2S x 9H2O as a 5% solution and autoclave under a N2 atmosphere. Aseptically and anaerobically add the sterile Na2S x 9H2O solution to the medium under a N2-CO2 (80:20) atmosphere, and readjust pH to 6.9, if necessary. Pressurize inoculated bottles to 200 kPa N2-CO2 (80:20). |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
MnSO4 x n H2O | 0.50 | g | 0.5 | - | |
NaCl | 1.00 | g | 1 | 17.112 | |
FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
CoSO4 x 7 H2O | 0.10 | g | 0.1 | 0.645 | |
CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
AlK(SO4)2 | 0.01 | g | 0.01 | 0.039 | |
H3BO3 | 0.01 | g | 0.01 | 0.162 | |
Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0. |
Last modified: | 22.02.22 |
Source: | JCM |
Taxonomic range: | Archaea |
Medium type: | Complex medium |
Final pH: | 6.9 |
Equipment needed: | Anaerobic workbench, Autoclave, Filter |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 18.01 | 308.179 |
NaHCO3 | 5 | 59.519 |
MgCl2 x 6 H2O | 4 | 19.675 |
MgSO4 x 7 H2O | 3.48 | 14.119 |
Sodium L-lactate | 1.5 | 13.386 |
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