Name: ARCHAEOGLOBUS PROFUNDUS MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. J214 #

Equipment needed: Anaerobic workbench Filter Autoclave

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
KCl	0.34	g	0.34	4.561
MgCl₂ x 6 H₂O	4.00	g	4	19.675
MgSO₄ x 7 H₂O	3.45	g	3.45	13.997
NH₄Cl	0.25	g	0.25	4.674
CaCl₂ x 2 H₂O	0.14	g	0.14	0.952
K₂HPO₄	0.14	g	0.14	0.804
NaCl	18.00	g	18	308.008
NaHCO₃	1.00	g	1	11.904
Na₂SO₄	2.70	g	2.7	19.009
Fe(NH₄)₂(SO₄)₂ x 6 H₂O	2.00	mg	0.002	-
(NH₄)₂Ni(SO₄)₂ x 6 H₂O	2.00	mg	0.002	0.005
Trace minerals	10.00	ml	-	-
Yeast extract (BD-Difco)	0.50	g	0.5	-
Sodium acetate	1.00	g	1	12.191
Resazurin	1.00	mg	1.0e-3	0.004
Na₂S x 9 H₂O	0.50	g	0.5	2.082
Distilled water	990.00	ml	-	-
1 Mix ingredients except Na₂S x 9H₂O and adjust pH to 6.9 with H₂SO₄. Sterilize by filtration under H₂-CO₂ (80:20) gas mixture. Neutralize Na₂S x 9H₂O solution and autoclave under a N₂ atmosphere. Aseptically and anaerobically add the sterile Na₂S x 9H₂O solution to the medium under a H₂-CO₂ (80:20) atmosphere and readjust pH to 6.9, if necessary. Pressurize inoculated bottle to 200 kPa H₂-CO₂ (80:20).

Trace minerals #

1000

Solution occurs in 97 other media (first defined in Medium J151).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Nitrilotriacetic acid	1.50	g	1.5	7.848
MgSO₄ x 7 H₂O	3.00	g	3	12.172
MnSO₄ x n H₂O	0.50	g	0.5	-
NaCl	1.00	g	1	17.112
FeSO₄ x 7 H₂O	0.10	g	0.1	0.36
CoSO₄ x 7 H₂O	0.10	g	0.1	0.645
CaCl₂ x 2 H₂O	0.10	g	0.1	0.68
ZnSO₄ x 7 H₂O	0.10	g	0.1	0.348
CuSO₄ x 5 H₂O	0.01	g	0.01	0.04
AlK(SO₄)₂	0.01	g	0.01	0.039
H₃BO₃	0.01	g	0.01	0.162
Na₂MoO₄ x 2 H₂O	0.01	g	0.01	0.041
Distilled water	1000.00	ml	-	-
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Last modified:	22.02.22
Source:	JCM
Taxonomic range:	Archaea
Medium type:	Complex medium
Final pH:	6.9
Equipment needed:	Anaerobic workbench, Autoclave, Filter

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