Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Marine medium/Synthetic seawater mix solution | 125.00 | ml | - | - | |
Trace minerals | 10.00 | ml | - | - | |
Fe(NH4)2(SO4)2 x 6 H2O | 2.00 | mg | 0.002 | - | |
NH4Cl | 0.25 | g | 0.25 | 4.674 | |
(NH4)2Ni(SO4)2 x 6 H2O | 2.00 | mg | 0.002 | 0.005 | |
Na2SeO4 | 0.10 | mg | 1.0e-4 | 5.3e-4 | |
Na2WO4 x 2 H2O | 0.10 | mg | 1.0e-4 | - | |
NaHCO3 | 2.20 | g | 2.2 | 26.188 | |
KH2PO4 | 0.07 | g | 0.07 | 0.514 | |
Yeast extract
(BD-Difco) |
0.50 | g | 0.5 | - | |
Na2S2O3 x 5 H2O | 1.00 | g | 1 | 4.029 | |
Distilled water | 865.00 | ml | - | - | |
1 Adjust pH to 7.0 with 1 N H2SO4, and sterilize by filtration. Dispense the medium into bottles under a N2-CO2-O2 (80:20:1) atmosphere. Pressurize inoculated bottles to 200 kPa N2-CO2-O2 (80:20:1). |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
NaCl | 47.15 | g | 47.15 | 806.81 |
MgCl2 x 6 H2O | 18.10 | g | 18.1 | 89.03 |
MgSO4 x 7 H2O | 7.00 | g | 7 | 28.4 |
CaCl2 x 2 H2O | 3.13 | g | 3.13 | 21.29 |
Na2SO4 | 3.24 | g | 3.24 | 22.811 |
Na2CO3 | 0.10 | g | 0.1 | 0.944 |
NaBr | 0.10 | g | 0.1 | 0.972 |
KBr | 80.00 | mg | 0.08 | 0.672 |
SrCl2 x 6 H2O | 72.00 | mg | 0.072 | 0.27 |
H3BO3 | 52.00 | mg | 0.052 | 0.841 |
Na2HPO4 | 8.10 | mg | 0.008 | 0.057 |
NaF | 2.40 | mg | 0.002 | 0.057 |
Sodium silicate | 0.40 | mg | 4.0e-4 | 0.003 |
KI | 0.05 | mg | 5.0e-5 | 1.9e-4 |
Distilled water | 1000.00 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
MnSO4 x n H2O | 0.50 | g | 0.5 | - | |
NaCl | 1.00 | g | 1 | 17.112 | |
FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
CoSO4 x 7 H2O | 0.10 | g | 0.1 | 0.645 | |
CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
AlK(SO4)2 | 0.01 | g | 0.01 | 0.039 | |
H3BO3 | 0.01 | g | 0.01 | 0.162 | |
Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0. |
Last modified: | 22.02.22 |
Source: | JCM |
Taxonomic range: | Archaea |
Medium type: | Complex medium |
Final pH: | 7.0 |
Equipment needed: | Filter |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 5.904 | 101.022 |
MgCl2 x 6 H2O | 2.263 | 11.129 |
NaHCO3 | 2.2 | 26.188 |
Na2S2O3 x 5 H2O | 1 | 4.029 |
MgSO4 x 7 H2O | 0.905 | 3.672 |
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