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JCM 221 . THERMOANAEROBACTER ETHANOLICUS MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J221 #

ml
Equipment needed: Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 1.50 g 1.491 10.957
Na2HPO4 x 12 H2O 4.20 g 4.175 29.41
NH4Cl 0.50 g 0.497 9.292
MgCl2 x 6 H2O 0.18 g 0.179 0.88
Yeast extract
(BD-Difco) 		2.00 g 1.988 -
Glucose 8.00 g 7.952 44.14
Vitamin solution 0.50 ml - -
Wolfe's modified mineral elixir 5.00 ml - -
Resazurin 1.00 mg 9.9e-4 0.004
Reducing solution 40.00 ml - -
Distilled water 960.00 ml - -
1 Mix ingredients, except reducing solution, and bring to the boil while gassing with N2-H2 (95:5) gas mixture. When the color turns from blue to reddish pink, add the reducing solution during continuous gassing and boiling. Anaerobically dispense the medium in tubes under a N2-H2 (95:5) atmosphere. Stopper the tubes and autoclave.

Vitamin solution #

500
Solution occurs in 1 other media (first defined in Medium J221).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.04 0.164
p-Aminobenzoic acid 50 mg 0.1 0.729
Folic acid 20 mg 0.04 0.091
Calcium pantothenate 50 mg 0.1 0.21
Nicotinic acid 50 mg 0.1 0.812
Vitamin B12 1 mg 0.002 0.001
Thiamine HCl 5 mg 0.01 -
Pyridoxine hydrochloride 100 mg 0.2 -
Thioctic acid 50 mg 0.1 0.485
Riboflavin 5 mg 0.01 0.027
Distilled water 500 ml - -
1 Store at -20C or 4C in the absence of light.

Wolfe's modified mineral elixir #

1000
Solution occurs in 1 other media (first defined in Medium J221).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x n H2O 0.50 g 0.5 -
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
Co(NO3)2 x 6 H2O 0.10 g 0.1 0.344
CaCl2 0.10 g 0.1 0.901
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 0.01 g 0.01 0.039
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
Na2SeO3 1.00 mg 1.0e-3 -
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then proceed to add minerals in the order listed.

Reducing solution #

200
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH
(0.2 N) 		200.0 ml - -
Na2S x 9 H2O 2.5 g 12.5 52.044
L-Cysteine HCl x H2O 2.5 g 12.5 -
1 Boil NaOH solution and cool down under a N2-H2 (95:5) atmosphere, then add Na2S x 9H2O and L-cysteine x HCl x H2O to the solution. Autoclave it under a N2-H2 (95:5) atmosphere.

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.5
Equipment needed: Anaerobic workbench, Autoclave
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Associated strains

All strains for this medium: 6

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Glucose 7.952 44.14
Na2HPO4 x 12 H2O 4.175 29.41
Yeast extract 1.988 -
KH2PO4 1.491 10.957
NH4Cl 0.497 9.292
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Cultivation metadata from 6 strains BacDive

phyla 1 6 strains
oxygen anaerobe 6 strains
temperature thermophilic 6 strains