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J229. JCM MEDIUM No. 229

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 100.0 g 98.039 1677.53
2 1.0 g 0.98 13.151
3 2.0 g 1.961 7.955
4 10.0 g 9.804 54.419
5
(BD-Difco)
5.0 g 4.902 -
6
(BD-Difco)
5.0 g 4.902 -
7 0.5 g 0.49 3.71
8 1.0 g 0.98 5.582
9 Trace mineral solution 10.0 ml - -
10 Trace vitamins 10.0 ml - -
11 1.0 mg 9.8e-4 0.004
12 20.0 g 19.608 185.001
13 1000.0 ml 978.634 54322.4
Instructions:
  1. Check final pH to be about 10.0.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.50 g 1.5 7.848
2 3.00 g 3 12.171
3 0.50 g 0.5 -
4 1.00 g 1 17.111
5 0.10 g 0.1 0.36
6 0.10 g 0.1 -
7 0.10 g 0.1 0.68
8 0.10 g 0.1 -
9 0.01 g 0.01 -
10 0.01 g 0.01 0.039
11 0.01 g 0.01 0.162
12 0.01 g 0.01 0.041
13 1000.00 ml 998.207 55408.8
Instructions:
  1. Dissolve nitrilotriacetic acid with KOH to pH 6.5, then proceed to add minerals.
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
NaCl 98.049 1677.7
Na2CO3 19.608 185.001
Glucose 9.804 54.419
Yeast extract 4.902 -
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