Name: JCM MEDIUM No. 229
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. J229 #

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
NaCl	100.0	g	98.039	1677.6
KCl	1.0	g	0.98	13.151
MgSO₄ x 7 H₂O	2.0	g	1.961	7.955
Glucose	10.0	g	9.804	54.418
Tryptone (BD-Difco)	5.0	g	4.902	68.964
Yeast extract (BD-Difco)	5.0	g	4.902	-
(NH₄)₂SO₄	0.5	g	0.49	3.71
L-Cysteine HCl x H₂O	1.0	g	0.98	-
Trace mineral solution	10.0	ml	-	-
Trace vitamins	10.0	ml	-	-
Resazurin	1.0	mg	9.8e-4	0.004
Na₂CO₃	20.0	g	19.608	185.001
Distilled water	1000.0	ml	-	-
1 Check final pH to be about 10.0.

Trace mineral solution #

1000

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Nitrilotriacetic acid	1.50	g	1.5	7.848
MgSO₄ x 7 H₂O	3.00	g	3	12.172
MnSO₄ x n H₂O	0.50	g	0.5	-
NaCl	1.00	g	1	17.112
FeSO₄ x 7 H₂O	0.10	g	0.1	0.36
CoCl₂ x 6 H₂O	0.10	g	0.1	0.77
CaCl₂ x 2 H₂O	0.10	g	0.1	0.68
ZnSO₄ x 7 H₂O	0.10	g	0.1	0.348
CuSO₄ x 5 H₂O	0.01	g	0.01	0.04
AlK(SO₄)₂	0.01	g	0.01	0.039
H₃BO₃	0.01	g	0.01	0.162
Na₂MoO₄ x 2 H₂O	0.01	g	0.01	0.041
Distilled water	1000.00	ml	-	-
1 Dissolve nitrilotriacetic acid with KOH to pH 6.5, then proceed to add minerals.

1000

Solution occurs in 220 other media (first defined in Medium J197).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Biotin	2.0	mg	0.002	0.008
Folic acid	2.0	mg	0.002	0.005
Pyridoxine hydrochloride	10.0	mg	0.01	0.049
Thiamine HCl	5.0	mg	0.005	0.015
Riboflavin	5.0	mg	0.005	0.013
Nicotinic acid	5.0	mg	0.005	0.041
Calcium pantothenate	5.0	mg	0.005	0.01
Vitamin B₁₂	0.1	mg	1.0e-4	7.4e-5
p-Aminobenzoic acid	5.0	mg	0.005	0.036
Lipoic acid	5.0	mg	0.005	0.024
Distilled water	1000.0	ml	-	-

All strains for this medium:

No strains found

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