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J231. METHANOBACTERIUM THERMOAUTOTROPHICUM MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.30 g 0.3 2.205
2 1.50 g 1.5 11.352
3 0.60 g 0.6 10.267
4 0.12 g 0.12 0.487
5 0.08 g 0.08 0.544
6 4.00 mg 0.004 0.014
7 0.15 g 0.15 0.861
8 4.00 g 4 37.74
9 Trace vitamins 10.00 ml - -
10 Trace element solution 10.00 ml - -
11 0.50 g 0.5 2.847
12 0.50 g 0.5 2.082
13 1.00 mg 1.0e-3 0.004
14 980.00 ml 978.243 54300.7
Instructions:
  1. Mix ingredients, except cysteine x HCl and Na2S x 9H2O, and adjust pH to 7.2. Bring to a boil for 5-10 sec, then, cool down under a stream of H2-CO2 (80:20). Dispense the medium into suitable culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) under a stream of H2-CO2 (80:20) and seal with butyl rubber stoppers. After autoclaving, stand the medium overnight. Separately autoclave cysteine x HCl and Na2S x 9H2O as 5% solutions, respectively, under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add the cysteine x HCl and Na2S x 9H2O solutions. Pressurize the inoculated bottles to 100 kPa H2-CO2 (80:20).
Solution occurs in 2 other media (first defined in medium J231 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.640 g - -
2 6.200 g - -
3 0.550 g - -
4 1.000 g - -
5 0.100 g - -
6 0.170 g - -
7 0.130 g - -
8 0.180 g - -
9 0.050 g - -
10 0.018 g - -
11 0.010 g - -
12 0.011 g - -
13 0.025 g - -
Instructions:
  1. Dissolve EDTA x 2Na first in 500 ml of distilled water. Then add the remaining salts and adjust volume to 1000 ml.
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 988.225 54854.8
Na2CO3 4 37.74
(NH4)2SO4 1.5 11.352
NaCl 0.6 10.267
L-Cysteine x HCl x H2O 0.5 2.847
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