Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KH2PO4 | 0.30 | g | 0.3 | 2.204 | |
(NH4)2SO4 | 1.50 | g | 1.5 | 11.352 | |
NaCl | 0.60 | g | 0.6 | 10.267 | |
MgSO4 x 7 H2O | 0.12 | g | 0.12 | 0.487 | |
CaCl2 x 2 H2O | 0.08 | g | 0.08 | 0.544 | |
FeSO4 x 7 H2O | 4.00 | mg | 0.004 | 0.014 | |
K2HPO4 | 0.15 | g | 0.15 | 0.861 | |
Na2CO3 | 4.00 | g | 4 | 37.74 | |
Trace vitamins | 10.00 | ml | - | - | |
Trace element solution | 10.00 | ml | - | - | |
L-Cysteine HCl x H2O | 0.50 | g | 0.5 | - | |
Na2S x 9 H2O | 0.50 | g | 0.5 | 2.082 | |
Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
Distilled water | 980.00 | ml | - | - | |
1 Mix ingredients, except cysteine x HCl and Na2S x 9H2O, and adjust pH to 7.2. Bring to a boil for 5-10 sec, then, cool down under a stream of H2-CO2 (80:20). Dispense the medium into suitable culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) under a stream of H2-CO2 (80:20) and seal with butyl rubber stoppers. After autoclaving, stand the medium overnight. Separately autoclave cysteine x HCl and Na2S x 9H2O as 5% solutions, respectively, under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add the cysteine x HCl and Na2S x 9H2O solutions. Pressurize the inoculated bottles to 100 kPa H2-CO2 (80:20). |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Na2-EDTA | 0.640 | g | - | - | |
MgSO4 x 7 H2O | 6.200 | g | - | - | |
MnSO4 x n H2O | 0.550 | g | - | - | |
NaCl | 1.000 | g | - | - | |
FeSO4 x 7 H2O | 0.100 | g | - | - | |
CoCl2 x 6 H2O | 0.170 | g | - | - | |
CaCl2 x 2 H2O | 0.130 | g | - | - | |
ZnSO4 x 7 H2O | 0.180 | g | - | - | |
CuSO4 x 5 H2O | 0.050 | g | - | - | |
AlK(SO4)2 x 12 H2O | 0.018 | g | - | - | |
H3BO3 | 0.010 | g | - | - | |
Na2MoO4 x 2 H2O | 0.011 | g | - | - | |
NiCl2 x 6 H2O | 0.025 | g | - | - | |
1 Dissolve EDTA x 2Na first in 500 ml of distilled water. Then add the remaining salts and adjust volume to 1000 ml. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 2.0 | mg | 0.002 | 0.008 |
Folic acid | 2.0 | mg | 0.002 | 0.005 |
Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
Riboflavin | 5.0 | mg | 0.005 | 0.013 |
Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Last modified: | 22.02.22 |
Source: | JCM |
Taxonomic range: | Archaea |
Medium type: | Defined medium |
Final pH: | 7.2 |
Equipment needed: | Anaerobic workbench, Autoclave |
Edit in Medium builder |
All strains for this medium: | 3 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Na2CO3 | 4 | 37.74 |
(NH4)2SO4 | 1.5 | 11.352 |
NaCl | 0.6 | 10.267 |
Na2S x 9 H2O | 0.5 | 2.082 |
L-Cysteine HCl x H2O | 0.5 | - |
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