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JCM 232 . METHANOCALDOCOCCUS JANNASCHII MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J232 #

ml
Equipment needed: Anaerobic workbench Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 0.14 g 0.14 0.804
CaCl2 x 2 H2O 0.14 g 0.14 0.952
NH4Cl 0.25 g 0.25 4.674
MgSO4 x 7 H2O 3.40 g 3.4 13.794
MgCl2 x 6 H2O 2.70 g 2.7 13.281
KCl 0.33 g 0.33 4.427
NiCl2 x 6 H2O 0.75 mg 7.5e-4 0.006
Na2SeO3 x 5 H2O 0.50 mg 5.0e-4 0.002
NaCl 30.00 g 30 513.347
Fe(NH4)2(SO4)2 x 6 H2O 0.01 g 0.01 -
Trace minerals 10.00 ml - -
Trace vitamins 10.00 ml - -
NaHCO3 1.00 g 1 11.904
L-Cysteine HCl x H2O 0.50 g 0.5 -
Na2S x 9 H2O 0.50 g 0.5 2.082
Resazurin 1.00 mg 1.0e-3 0.004
Distilled water 980.00 ml - -
1 Mix ingredients, except NaHCO3, cysteine x HCl and Na2S x 9H2O, and adjust pH to 6.0. Bring to a boil for 5-10 sec, then, cool down under a stream of H2-CO2 (80:20) and add NaHCO3 to the medium. Dispense the medium into suitable culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) under a stream of H2-CO2 (80:20) and seal with butyl rubber stoppers. After autoclaving, stand the medium overnight. Separately autoclave cysteine x HCl and Na2S x 9H2O as 5% solutions, respectively, under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add the cysteine x HCl and Na2S x 9H2O solutions. Pressurize the inoculated bottles to 200 kPa H2-CO2 (80:20).

Trace minerals #

1000
Solution occurs in 97 other media (first defined in Medium J151).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x n H2O 0.50 g 0.5 -
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.10 g 0.1 0.645
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 0.01 g 0.01 0.039
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Archaea
Medium type: Defined medium
Final pH: 6.0
Equipment needed: Anaerobic workbench, Autoclave
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Associated strains

All strains for this medium: 8

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 30.01 513.518
MgSO4 x 7 H2O 3.43 13.916
MgCl2 x 6 H2O 2.7 13.281
NaHCO3 1 11.904
Na2S x 9 H2O 0.5 2.082
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Cultivation metadata from 8 strains BacDive

phyla 1 8 strains
oxygen anaerobe 8 strains
temperature hyperthermophilic 6 strains
temperature thermophilic 2 strains