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JCM 237 . THERMOTOGA MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J237 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Starch
(soluble) 		5.0 g 4.926 -
KH2PO4 0.5 g 0.493 3.62
Trace minerals 15.0 ml - -
NiCl2 x 6 H2O 2.0 mg 0.002 0.015
NaCl 20.0 g 19.704 337.174
Yeast extract
(BD-Difco) 		0.5 g 0.493 -
Resazurin 1.0 mg 9.9e-4 0.004
Na2S x 9 H2O 0.5 g 0.493 2.051
Artificial sea water 250.0 ml - -
Distilled water 750.0 ml - -
1 Mix components except Na2S x 9H2O, adjust pH to 6.5 and autoclave under a N2 atmosphere. Separately autoclave a 5% Na2S x 9H2O solution (pH 7.0) under a N2 atmosphere. Prior to inoculation, add the sterile Na2S x 9H2O solution to the medium.

Artificial sea water #

1000
Solution occurs in 4 other media (first defined in Medium 343).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 27.70 g 27.7 473.99
MgSO4 x 7 H2O 7.00 g 7 28.4
MgCl2 x 6 H2O 5.50 g 5.5 27.053
KCl 0.65 g 0.65 8.719
NaBr 0.10 g 0.1 0.972
H3BO3 30.00 mg 0.03 0.485
SrCl2 x 6 H2O 15.00 mg 0.015 0.056
Citric acid 10.00 mg 0.01 0.052
KI 0.05 mg 5.0e-5 1.9e-4
CaCl2 x 2 H2O 2.25 g 2.25 15.305
Distilled water 1000.00 ml - -

Trace minerals #

1000
Solution occurs in 97 other media (first defined in Medium J151).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x n H2O 0.50 g 0.5 -
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.10 g 0.1 0.645
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 0.01 g 0.01 0.039
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.5
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 26.542 454.173
Starch 4.926 -
MgSO4 x 7 H2O 1.768 7.175
MgCl2 x 6 H2O 1.355 6.663
CaCl2 x 2 H2O 0.556 3.78
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature hyperthermophilic 1 strain